Pressure-dependent inverse bicontinuous cubic phase formation in a phosphatidylinositol 4-phosphate/phosphatidylcholine system

doi:10.1016/j.cdc.2016.08.001

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Volumes 3–4, August 2016, Pages 15-20

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Abstract

In this paper, we report the inositide-driven formation of an inverse bicontinuous cubic phase with space group Ia3d (Q_II^G, gyroid phase). The system under study consisted of distearoylphosphatidylinositol 4-phosphate (DSPIP) and dioleoylphosphatidylcholine at a molar ratio of 1:49, with a physiological concentration of magnesium ions at pH 7·4. The behaviour of the system was monitored as a function of temperature and pressure. The formation of the phase with Ia3d geometry was recorded repeatably at high pressure, and occurred more readily at higher temperatures. We conclude that the Ia3d phase formed is a thermodynamically stable structure, and that DSPIP is a potent source of membrane curvature that can drive the formation of mesophases with both 2- and 3D geometry.

Section snippets

Rationale

Research interest in lipid aggregations with cubic geometry has increased in recent years, owing to suggestions that swollen cubic phases are a model for the membrane division events observed in vivo. Such events include endocytosis [1], [2], [3], vesicle formation [4], [5] and vesicle trafficking [6]. Work on model lipid systems has provided evidence for induction of vesicle budding with changes in buffer [7] and for cubic phases that are hydration-dependent [8] and tuneable with respect to

Procedure

DOPC was obtained from Avanti Polar Lipids (Alabaster, AL) and used without further purification. All salts, solvents and buffers were obtained from Sigma Aldrich (St. Louis, MO). Racemic DSPIP was prepared in our laboratory [19], [20], as its bis-ammonium salt.

Data, value and validation

In order to explore the potential for inositides to induce membrane curvature, we have studied a lipid mixture comprising distearoyl phosphatidylinositol 4-phosphate (DSPIP, a PI-4-P) and dioleoylphosphatidylcholine (DOPC) (molar ratio 1:49) as a function of pressure and temperature. This binary system was hydrated using excess buffer (5 mM MgCl₂ and 20 mM Tris-base, adjusted to pH 7·4 at 25 °C). Both the proportion of DSPIP and the magnesium ion concentration are typical of those found in

Author contributions

The experimental work was designed and carried out by SF and NJB, and supervised by RHT. RW, RHT and PRJG conceived the research project and wrote the grant application. The manuscript was written by SF and NJB. All authors commented on the manuscript and approved its final form.

Acknowledgements

SF would like to thank Prof. O. Ces and Dr G. C. Shearman for excellent guidance, support and helpful discussions. This work was supported by ESPRC Platform grant GR/S77721, BBSRC Grant BB/F013167/1 and by an EPSRC Centre for Doctoral Training Studentship from the Institute of Chemical Biology (Imperial College London) awarded to SF. We acknowledge the European Synchrotron Radiation Facility (Grenoble, France) for the provision of synchrotron radiation facilities and we would like to thank Dr

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Data article Pressure-dependent inverse bicontinuous cubic phase formation in a phosphatidylinositol 4-phosphate/phosphatidylcholine system

Abstract

Section snippets

Rationale

Procedure

Data, value and validation

Author contributions

Acknowledgements

J. Biol. Chem.

J. Lipid Res.

Cell

Org. Biomol. Chem.

Curr. Biol.

Biochim. Biophys. Acta

Nature

Biochem. J.

Plant Cell

Mol. Biol. Cell

Langmuir

Soft Matter

Soft Matter

Soft Matter

Langmuir

Data article
Pressure-dependent inverse bicontinuous cubic phase formation in a phosphatidylinositol 4-phosphate/phosphatidylcholine system