Data and code availability
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The EM image data, cellular segmentation, synapse segmentation, mitochondrion segmentation, and nucleus segmentation can be viewed at https://microns-explorer.org/phase1. The raw calcium imaging videos, light microscopy coregistration volume, visual stimulus, and calcium traces can be accessed via https://microns-explorer.org/phase1, and these are also available at https://github.com/seung-lab/MicronsBinder with the analysis code. Both the MicronsExplorer and MicronsBinder websites demonstrate how to access all intermediate analysis data (deposited as a Zenodo record).
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Along with the analysis code repository (https://github.com/seung-lab/MicronsBinder), the code for generating the cell and organelle reconstructions is available at https://github.com/seung-lab/.
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All original code has been deposited at Zenodo and is publicly available as of the date of publication. DOIs are listed in the key resources table.
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Any additional information required to reanalyze the data reported in this paper is available from the lead contact upon request.