Cell Host & Microbe
Volume 14, Issue 1, 17 July 2013, Pages 38-50
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Article
The Legionella Effector RidL Inhibits Retrograde Trafficking to Promote Intracellular Replication

https://doi.org/10.1016/j.chom.2013.06.001Get rights and content
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Highlights

  • The host retromer complex restricts Legionella replication and localizes to LCVs

  • The Legionella effector RidL targets the eukaryotic retromer Vps29 subunit

  • Ectopic production of RidL inhibits retrograde transport in eukaryotic cells

  • Deletion of ridL abrogates the inhibition of retrograde trafficking by Legionella

Summary

The bacteria causing Legionnaires’ disease, Legionella pneumophila, replicate intracellularly within unique Legionella-containing vacuoles (LCVs). LCV formation involves a type IV secretion system (T4SS) that translocates effector proteins into host cells. We show that the T4SS effector RidL localizes to LCVs, supports intracellular bacterial growth, and alters retrograde trafficking, in which selected proteins are transported from endosomes to the Golgi. The retromer complex that mediates retrograde trafficking localizes to LCVs independently of RidL and restricts intracellular bacterial growth. RidL binds the Vps29 retromer subunit and the lipid PtdIns(3)P, which localizes retromer components to membranes. Additionally, specific retromer cargo receptors and sorting nexins that mediate protein capture and membrane remodeling preferentially localize to LCVs in the absence of ridL. Ectopic RidL production inhibits retrograde trafficking, and L. pneumophila blocks retrograde transport at endosome exit sites in a ridL-dependent manner. Collectively, these findings suggest that RidL inhibits retromer function to promote intracellular bacterial replication.

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Present address: F. Hoffmann-La Roche Ltd., PTDSC2H, 4070 Basel, Switzerland

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These authors contributed equally to this work