J Appl Biomed 16:328-336, 2018 | DOI: 10.1016/j.jab.2018.05.003

A proteomic glimpse into the oncogenesis of prostate cancer

Patrícia Borba Martiny^a,*, Diego Duarte Alcoba^a, Brasil Silva Neto^b, Paulo Costa Carvalho^c, Ilma Simoni Brum^a

^a Universidade Federal do Rio Grande do Sul, Institute of Health Sciences, Department of Physiology, Porto Alegre, Brazil

^b Hospital de Clínicas de Porto Alegre, Service of Urology, Porto Alegre, Brazil

^c Carlos Chagas Institute, Laboratory of Proteomics and Protein Engineering, Fiocruz, Paraná, Brazil

Prostate cancer (PCa) is the second most frequent cancer in men worldwide. Distinguishing between the nonaggressive and aggressive forms of this disease is difficult, and a means to better characterize molecular patterns that could aid in diagnosis is urgently needed. Here, we compare the proteomic profiles of PCa and benign prostatic hyperplasia (BPH) in an effort to elucidate underlying mechanisms of oncogenesis. We compared protein expression in PCa and BPH tissue biopsies using quantitative tandem mass tag (TMT) and a MultiNotch data acquisition proteomic on an Orbitrap Fusion. Four proteins that were observed to be differentially abundant in the mass spectrometry analysis were selected for further comparison with quantitative real-time PCR: S100A4, L-lactate dehydrogenase B-chain (LDHB), Phosphatidylethanolamine-binding-protein 1-RAF (RKIP), and Ras suppressor-protein-1 (RSU1). Mass spectrometry showed RKIP and RSU1 to be upregulated in PCa, while S100A4 and LDHB were upregulated in BPH. q-PCR results were in agreement with quantitative proteomic data in BPH tissue but disagree with gene expression analysis of PCa samples. These four elements showed higher gene expression in BPH than in PCa. Taken together, our results complement and reinforce the current understanding of prostate cancer progression.

Keywords: Prostate cancer; Benign prostatic hyperplasia; Proteomics; Tandem mass tag; Mass spectrometry

Received: June 7, 2017; Revised: April 24, 2018; Accepted: May 16, 2018; Published: November 1, 2018  Show citation