Advanced glycation end products in idiopathic frozen shoulders
Section snippets
Study design
A prospective case-control study was conducted at the Orthopaedics Research Institute, St. George Hospital, and the National Day Surgery, Sydney, NSW, Australia, during a 9-month period.
Inclusion and exclusion criteria
The study included patients with primary frozen shoulders who were receiving arthroscopic capsular releases. It also included patients with unstable shoulders undergoing arthroscopic stabilization and patients with rotator cuff tears undergoing rotator cuff repair as control groups. Instability and rotator cuff
Patient demographics
The study included 8 patients (6 nondiabetic and 2 diabetic patients) with frozen shoulder as the disease population. Three male and 5 female frozen shoulder patients had a mean age of 52 years (range, 41-60). Six shoulder instability patients (5 male and 1 female) had a mean age of 23 years (range, 16-40). Eight rotator cuff tear patients (5 male and 3 female) had a mean age of 58 years (range, 52-71) in the control populations. The mean duration of symptoms before shoulder surgeries was 5
Discussion
This study has found increased immunoreactivity of AGEs in frozen shoulder capsules compared with control capsules. Histologic features of all H&E-stained frozen shoulder capsule slides included densely packed collagen fibers, fibroblastic hypercellularity, and increased vascularization. In addition, increased numbers of adipocytes were noted in frozen shoulder capsules compared with control capsules.
Our study used tissue from shoulder conditions other than frozen shoulder because it would have
Conclusion
This study highlights a potential role for AGEs in the pathogenesis of frozen shoulder. As far as we can determine, this paper is the first to consider whether AGEs are involved in the frozen shoulders of patients regardless of their diabetic status. In addition, we have also found increased numbers of adipocytes in the capsular tissues of frozen shoulder patients compared with capsular tissues of rotator cuff tears and shoulder instability patients. The overexpression of AGEs may explain the
Disclaimer
The authors, their immediate families, and any research foundation with which they are affiliated have not received any financial payments or other benefits from any commercial entity related to the subject of this article.
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2021, Journal of Bodywork and Movement TherapiesCitation Excerpt :Frozen shoulder (FS) is one of the most common shoulder conditions, characterized by pain, stiffness and restricted active and passive range of motion. Prevalence ranges from 2% to 5% in general population (Hwang et al., 2016; Kelley et al., 2013). It is relatively more common in women than men, often in non-dominant arm and age between 40 and 60 years.
Inhibitory mechanism of sinensetin on α-glucosidase and non-enzymatic glycation: Insights from spectroscopy and molecular docking analyses
2021, International Journal of Biological MacromoleculesCitation Excerpt :Under normal physiological conditions, the formation of AGEs is usually slow [12]. However, longstanding hyperglycemic state in diabetes can accelerate their formation [13]. AGEs play a significant role in the occurrence and development of diabetes complications, and they are also the pathophysiological base of many neurodegenerative diseases like cardiovascular diseases and Alzheimer's disease [14,15].
Association between fasting glucose levels and adhesive capsulitis in a normoglycemic population: a case-control study
2020, Journal of Shoulder and Elbow SurgeryCitation Excerpt :The accumulation of hyperglycemia-induced advanced glycation end products (AGEs) and the consequent cross-linking of collagen have been suggested to contribute to the higher incidence of adhesive capsulitis in diabetes.10 Hwang et al25 reported an increase in AGEs in both diabetic and nondiabetic patients with adhesive capsulitis, as compared with instability patients or patients with rotator cuff tear, suggesting that the accumulation of AGEs potentially contributes to the pathogenesis of adhesive capsulitis, regardless of diabetic status. Most studies that have evaluated hyperglycemic effects on IAC have focused on diabetes.
RAGE-dependent NF-kB inflammation processes in the capsule of frozen shoulders
2020, Journal of Shoulder and Elbow SurgeryCitation Excerpt :The discrepancy in results between their study and our own might be due to the difference in the type of samples collected. Finally, although Hwang et al21 found that AGE overexpression causes fibroblastic proliferation in FS, details regarding the specific AGEs involved were not reported. Cardiovascular injury or diabetic hyperglycemia leads to the storage of AGEs, such as CML, in vascular endothelial cells, and AGEs bind to RAGEs on these cells,3,28,45 triggering the activation of NF-kB by interacting with mitogen-activated protein kinase and extracellular signal-regulated kinase signaling pathways.7,27
Aberrant structure of fibrillar collagen and elevated levels of advanced glycation end products typify delayed fracture healing in the diet-induced obesity mouse model
2020, BoneCitation Excerpt :To detect AGEs accumulation in the fracture callus, we performed IF staining using a widely used polyclonal antibody that detects a wide range of AGEs [43–45] (further details are provided in Supplementary Table 1). The ability of this antibody to detect AGEs accumulation as a result of aging or disease conditions has been validated in different tissues, including musculoskeletal tissues [18,46–52]. Given that AGEs modify several proteins, and that our aim is to detect AGEs accumulation in Col I, we co-stained AGEs and Col I and limited our analysis to areas where they colocalize as detected by confocal microscopy (Supplementary Fig. S7).
The study was conducted in accordance with ethics approval from the Human Research Ethics Committee—Central Network, South East Health (HREC/96/55, HREC/14/130).