Elsevier

NeuroImage

Volume 49, Issue 2, 15 January 2010, Pages 1728-1740
NeuroImage

The image of time: A voxel-wise meta-analysis

https://doi.org/10.1016/j.neuroimage.2009.09.064Get rights and content

Abstract

Although there has been an explosion of interest in the neural correlates of time perception during the past decade, substantial disagreement persists regarding the structures that are relevant to interval timing. We addressed this important issue by conducting a comprehensive, voxel-wise meta-analysis using the activation likelihood estimation algorithm; this procedure models each stereotactic coordinate as a 3D Gaussian distribution, then tests the likelihood of activation across all voxels in the brain (Turkeltaub et al., 2002). We included 446 sets of activation foci across 41 studies of timing that report whole-brain analyses. We divided the data set along two dimensions: stimulus duration (sub- vs. supra-second) and nature of response (motor vs. perceptual).

Our meta-analyses revealed dissociable neural networks for the processing of duration with motor or perceptual components. Sub-second timing tasks showed a higher propensity to recruit sub-cortical networks, such as the basal ganglia and cerebellum, whereas supra-second timing tasks were more likely to activate cortical structures, such as the SMA and prefrontal cortex. We also detected a differential pattern of activation likelihood in basal ganglia structures, depending on the interval and task design. Finally, a conjunction analysis revealed the SMA and right inferior frontal gyrus as the only structures with significant voxels across all timing conditions. These results suggest that the processing of temporal information is mediated by a distributed network that can be differentially engaged depending on the task requirements.

Introduction

Numerous neuroimaging studies investigating the neural correlates of time perception in the range of milliseconds to minutes have been published in the last decade. The results of these studies have led to a number of different theories about the roles of particular neural structures in time perception. For example, some investigators have emphasized the role of the basal ganglia in temporal processing (e.g., Buhusi and Meck, 2005), whereas others suggest a preferential role of the right prefrontal cortex (Lewis and Miall, 2006a, Lewis and Miall, 2006b). Still other accounts have assigned essential functions to the cerebellum (Ivry et al., 2002), supplementary motor area (SMA; Macar et al., 2006), right inferior parietal lobe (IPL; Bueti and Walsh, 2009), and insular cortex (Craig, 2009). Substantial differences in the nature of the tasks employed, the duration of the stimuli to be timed, the nature of the response and the baseline or control condition complicate comparisons between these studies and may account for the lack of consistency. In order to fully characterize the manner in which brain activity relates to temporal perception, quantitative methods may be necessary to search for concordance among neuroimaging studies. Here we present a quantitative, voxel-wise meta-analysis of neuroimaging data designed to determine regions of concordance in the current literature on time perception.

Several factors may contribute to the variability in imaging studies reported to date. One factor may be the wide variety of tasks that have been employed. A dimension on which timing tasks vary is the degree to which they engage “motor” or “perceptual” timing routines. For motor timing tasks, the interval to be timed is defined by a motor response. The most commonly used task among motor timing studies is paced finger tapping, in which subjects are typically required to tap a response key in time with an isochronous metronome at a given rate (synchronization), and then continue tapping at the same rate without any external stimulation (continuation). In some studies, no continuation phase is employed, while in others the subject is required to tap at the midpoint between sets of pacing stimuli (syncopation). Another motor timing task is interval production/reproduction, in which subjects produce an interval from memory, by indicating in a stopwatch-like fashion when the interval begins and ends. A common variation in production/reproduction is the type of memory utilized, as investigators may require subject to produce an interval that was experienced recently (reproduction), or produce a prescribed duration (i.e., “produce 7 s”). Perceptual timing tasks, in contrast, require subjects to make judgments about temporal intervals. A task frequently employed in neuroimaging studies of perceptual timing is temporal discrimination, in which subjects compare the duration of successive stimuli by indicating whether the second stimulus is longer than or differs from the first.

A second factor that may contribute to variability is the duration of the stimulus to be timed. Neuroimaging studies have utilized test intervals ranging from 200 ms to 24 s. A number of distinct lines of evidence suggest that mechanisms and brain regions recruited in timing tasks differ as a function of the stimulus duration (Mauk and Buonomano, 2004, Buhusi and Meck, 2005, Lewis and Miall, 2003b). Evidence from neuropsychological studies in humans and investigations in animals suggest that timing of intervals above and below 1 s relies on different procedures (Harrington and Haaland, 1999, Buhusi and Meck, 2005, Gibbon et al., 1997). Lewis and Miall (2003b) suggested that sub-second intervals are embedded in motor action plans, while supra-second intervals require greater cognitive control. Additionally, Mauk and Buonomano (2004) suggest that the timing of sub-second stimuli may rely on sensory processes, wherein state-dependent networks may encode changes in duration (Karmarkar and Buonomano, 2007). Rammsayer (1997) proposed that timing of different durations may be dependent on different neurotransmitter systems; he reported, for example, that haloperidol, a non-specific D2 dopamine-receptor antagonist, altered timing performance in humans across both sub-second and supra-second ranges, whereas remoxipride, a D2 dopamine-receptor antagonist which primarily acts on mesolimbocortical projections (Gerlach and Casey, 1990), altered only supra-second performance.

Several reviews to date have attempted to characterize activations across the corpus of neuroimaging studies of timing. Macar and colleagues (2002) divided neuroimaging studies into rhythmic and perceptual categories; only activations resulting from control task subtractions were considered. The authors concluded that the basal ganglia (caudate and putamen), SMA, cerebellum, dorsolateral prefrontal cortex (DLPFC, Brodmann area [BA] 9/46), anterior cingulate and right IPL (BA 40) were often active across all timing tasks, suggesting that these regions formed the core network of time perception in the brain. However, in addition to studies employing positron emission tomography (PET) and functional magnetic resonance imaging (fMRI) techniques, the authors also included results from studies measuring surface Laplacians, electroencephalography (EEG) and scalp current densities. One potential weakness of the latter studies is that EEG and related techniques do not readily interrogate sub-cortical structures and may thus underestimate the role that these structures play in timing.

Lewis and Miall (2003b) reported a label-based meta-analysis of neuroimaging of timing. The authors considered three factors in partitioning the datasets for their analysis: interval duration (sub-second or supra-second intervals), nature of the response (motor vs. non-motor), and whether the motor response was continuous or discontinuous. The results taken from each study were from the least restrictive contrasts, the majority of which were [task – rest]. The results of their meta-analysis suggest different neural networks are recruited, depending on the task constraints. Sub-second tasks commonly activated the bilateral SMA, left sensorimotor cortex, basal ganglia and thalamus, as well as the right cerebellum, superior temporal gyrus and lateral premotor cortex. Supra-second tasks frequently activated the left cerebellum, as well as the bilateral prefrontal and parietal cortices, with the right DLPFC demonstrating more common activation than any other structure. Among all studies, the SMA and right cerebellum were the most commonly activated structures.

Two recent label-based reviews have also attempted to characterize common activations across timing tasks. Meck, Penney and Pouthas (2008) reviewed a number of neuroimaging studies, concluding that the basal ganglia serve as a core timer across sub-second and supra-second timing tasks. Penney and Vaitilingam (2008) reviewed a larger corpus of neuroimaging timing studies; their review also divided experiments into sub-second and supra-second categories. The authors concluded that sub-second timing tasks most commonly activated the cerebellum, DLPFC, inferior frontal gyrus (IFG), superior temporal gyrus, premotor cortex, insula (BA 13), cingulate, pre-SMA, SMA, basal ganglia and thalamus, with the cerebellum being the most frequently activated. Supra-second timing tasks commonly activated the IFG, DLPFC, supramarginal gyrus (SMG), superior temporal gyrus, premotor cortex, cingulate, pre-SMA, middle temporal gyrus, basal ganglia and thalamus, with the IFG being the most frequently activated. However, this review included both activations subtracted from control tasks and from rest; as subtractions from rest do not control for other cognitive processes, activations in these studies may be related to a variety of cognitive operations in addition to timing procedures. Furthermore, the authors did not include any imaging studies employing paced finger tapping in their review.

Although these reviews have generated important and provocative findings, inconsistency across the studies persists, perhaps reflecting the fact that different studies were included, and discrepant procedures were employed to identify sites and extents of activation. Finally, although label-based reviews and meta-analyses may provide an overview of neuroimaging results, they do not provide any quantitative measure of the probability that a structure will be activated (Laird et al., 2005b).

With the dramatic increase in functional neuroimaging studies, the need has arisen for quantitative methods to evaluate cross-study results. Activation likelihood estimation (ALE) is a powerful, well-validated technique for conducting meta-analyses that circumvents a number of problems inherent in previous techniques. Developed concurrently yet independently by Turkeltaub and others (2002) and Chein and others (2002), ALE is a quantitative technique for conducting a voxel-wise analysis of cross-study data.

The ALE technique involves several steps. First, data is pooled across the set of selected studies; the data consists of the activation peaks reported in each study. Next, each activation peak is modeled as a 3D Gaussian probability distribution in the brain; the width of the Gaussian models the uncertainty in localization. The overlap among these distributions is used to estimate the probability that at least one of the peaks from the literature should have fallen within a given voxel (ALE score). A permutation test is then conducted by computing voxel-wise ALE scores for randomly generated lists of activation peaks. The ALE scores from the literature analysis are compared to those from the random sets of foci to calculate the statistical significance of each ALE score. Since the inception of ALE, a number of improvements have been made to increase the flexibility and utility of the algorithm. Many of these improvements were borrowed from techniques used for the analysis of functional neuroimaging data, and include the introduction of false-discovery rate (FDR) thresholds and cluster analysis (Laird et al., 2005a).

While the ALE technique is a quantitative technique for characterizing large sets of neuroimaging data, several weaknesses also exist. First, the size and shape of activation clusters is not considered; the ALE algorithm determines the spread of activation as a user-defined full-width at half-maximum (FWHM) Gaussian. Second, the number of subjects tested in each study is not considered. Finally, the ALE technique does not consider the relative contribution of each study to each ALE value; because of this limitation, particularly strong data from a single study may identify voxels that are judged to be “significant” despite the fact that they are not activated in the vast majority of studies included in the meta-analysis. We attempt to correct for some of these weaknesses with a new masking technique, described in more detail below.

Section snippets

Activation likelihood estimation

The ALE algorithm models the probability that a particular focus will be located in a given voxel, using 3-dimensional Gaussian distributions. The probability that a focus should have been identified at a given location is calculated asP=e(d2/2σ2)(2π)3/2σ3where d is the Euclidean distance from the center of the voxel to the focus and σ is the standard deviation of a Gaussian distribution. The probability value, which ranges between 0 and 1, is multiplied by 8 mm3, to give the cumulative

Sub-second motor timing

High areas of concordance for tasks involving sub-second intervals, as evaluated by a motor task, were found in the bilateral SMA, left middle frontal gyrus (BA 6) and precentral gyrus, right putamen and lateral globus pallidus, left thalamus, claustrum and posterior cerebellum. Additional clusters were found in the right IFG (BA 47), left middle frontal gyrus (BA 10), right posterior cerebellum, right middle frontal gyrus (BA 6), substantia nigra, IPL (BA 40), left superior temporal gyrus,

Discussion

The results of our meta-analysis revealed a number of distinct regions associated with the processing of temporal intervals. Furthermore, the likelihood that particular structures were activated depended on the duration of the interval to be timed and whether the task required predominantly motor or perceptual processing. Only the right IFG and bilateral SMA demonstrated significant activation likelihood across all conditions. These results support a number of conclusions. First, partially

Conclusions

The present study provides the first voxel-wise meta-analysis of the neuroimaging literature on time perception. Although our findings reinforce a number a previous observations about the roles of brain structures such as the right IFG and the SMA, they provide a more nuanced perspective on the contributions of structures such as the cerebellum and basal ganglia to timing. Our results suggest that different neural regions are recruited between sub-second and supra-second intervals, with

Acknowledgement

The authors would like to thank Dr. Matthew Matell for his helpful comments on the data presented in this manuscript.

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