Trends in Biotechnology
ReviewSpecial Issue: Celebrating 30 years of biotechnologyExtracellular matrix scaffolds for cartilage and bone regeneration
Highlights
āŗ Extracellular matrix scaffolds provide a bioactive platform for tissue regeneration. āŗ Osteochondral defect repair remains a great orthopedic and socioeconomic burden. āŗ We describe cartilage and bone decellularization and possible future directions. āŗ Extracellular matrix scaffolds hold great promise for osteochondral defect repair.
Section snippets
The need for improved repair of osteochondral defects
Joint injuries are common in the young and active population and often result in cartilage or osteochondral lesions. If untreated, these defects lead to joint swelling, pain, and serious restrictions in daily activities and can eventually progress towards osteoarthritis (OA), of which the only end-stage, salvaging therapy is artificial joint replacement. Over 151 million people suffer from OA worldwide [1], representing a huge clinical and socioeconomic burden. Established OA is notoriously
ECM-based regenerative medicine
All tissues are composed of cells surrounded by ECM that consists of a unique and tissue-specific 3D environment of structural and functional molecules secreted by the resident cells [14]. There is reciprocal interaction between cells and ECM; cellular products, including proteinases, modify the ECM, and ECM-incorporated growth factors and cytokines act as functional cues, steering the metabolic and secretory activity of cells. This situation becomes even more complex because the intricate
Application of ECM-based scaffolds to treat osteochondral defects
Treatments during which osteochondral plugs, taken either from a non-load-bearing region of the joint (mosaicplasty) or from a donor (allogeneic osteochondral grafting), are used to fill the defect can theoretically be considered ECM-based strategies because they imply the direct implantation of cartilage and bone matrix (Figure 1). However, the use of seeded or unseeded ECM-based scaffolds is a new and emerging approach within the field of cartilage tissue engineering, supported by a slowly
Future perspectives for ECM-based scaffolds for osteochondral repair
The use of decellularized ECM is gaining ground within the field of cartilage tissue engineering and may prove to be of great potential because it allows for multifactorial mimicry that has not yet been achieved by man-made biomaterials. The approach is still relatively underexplored and extensive research is required to understand the biologic responses to ECM scaffolds within the joint environment and to optimize the decellularization techniques and ultimately the final repair tissue. There
Concluding remarks
ECM scaffolds have shown great promise within the field of tissue engineering and are now being developed specifically for cartilage repair. Decellularized ECM-based scaffolds may solve many problems associated with the matrix-based approaches currently used for the repair of cartilage or osteochondral defects, such as osteochondral allografting and mosaicplasty. This approach may lead to the development of the ideal cartilage or osteochondral scaffold, providing the injured site with the right
Glossary
- Allograft
- graft obtained from a donor of the same species.
- bFGF
- basic fibroblast growth factor; involved in angiogenesis, wound healing, and embryonic development.
- EGF
- epidermal growth factor; stimulates proliferation and differentiation.
- IGF
- insulin-like growth factor; regulates cellular proliferation and apoptosis.
- Mosaicplasty
- surgical procedure during which a defect is filled with osteochondral plugs taken from a non-load-bearing region of the joint.
- Osseous phase
- bone compartment.
- Proteoglycans
References (73)
- et al.
Osteochondral allograft transplantation
Clin. Sports Med.
(1999) Cartilage engineering: a crucial combination of cells, biomaterials and biofactors
Trends Biotechnol.
(2009)Atelocollagen-associated autologous chondrocyte implantation for the repair of chondral defects of the knee: a prospective multicenter clinical trial in Japan
J. Orthop. Sci.
(2009)The mechanical properties and cytotoxicity of cell-laden double-network hydrogels based on photocrosslinkable gelatin and gellan gum biomacromolecules
Biomaterials
(2012)Engineered whole organs and complex tissues
Lancet
(2012)Clinical transplantation of a tissue-engineered airway
Lancet
(2008)Scaffold devices for rotator cuff repair
J. Shoulder Elbow Surg.
(2012)The extracellular matrix as a biologic scaffold material
Biomaterials
(2007)Effects of extracellular matrix and neighboring cells on induction of human embryonic stem cells into retinal or retinal pigment epithelial progenitors
Exp. Eye Res.
(2008)Extracellular matrix-derived products modulate endothelial and progenitor cell migration and proliferation in vitro and stimulate regenerative healing in vivo
Matrix Biol.
(2010)