Elsevier

Developmental Biology

Volume 365, Issue 2, 15 May 2012, Pages 319-327
Developmental Biology

Regulation of zebrafish heart regeneration by miR-133

https://doi.org/10.1016/j.ydbio.2012.02.018Get rights and content
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Abstract

Zebrafish regenerate cardiac muscle after severe injuries through the activation and proliferation of spared cardiomyocytes. Little is known about factors that control these events. Here we investigated the extent to which miRNAs regulate zebrafish heart regeneration. Microarray analysis identified many miRNAs with increased or reduced levels during regeneration. miR-133, a miRNA with known roles in cardiac development and disease, showed diminished expression during regeneration. Induced transgenic elevation of miR-133 levels after injury inhibited myocardial regeneration, while transgenic miR-133 depletion enhanced cardiomyocyte proliferation. Expression analyses indicated that cell cycle factors mps1, cdc37, and PA2G4, and cell junction components cx43 and cldn5, are miR-133 targets during regeneration. Using pharmacological inhibition and EGFP sensor interaction studies, we found that cx43 is a new miR-133 target and regeneration gene. Our results reveal dynamic regulation of miRNAs during heart regeneration, and indicate that miR-133 restricts injury-induced cardiomyocyte proliferation.

Highlights

► Functionally dissected miRNA contributions during cardiac regeneration. ► Developed inducible transgenic technology to modulate miRNA function. ► Modulation of miR-133 toggles between cardiac regeneration and scar formation. ► Identified cx43 as new cardiac regeneration gene and direct target of miR-133.

Keywords

Zebrafish
Heart
Regeneration
MicroRNAs
miR-133

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