Elsevier

Methods

Volume 68, Issue 1, 15 June 2014, Pages 74-81
Methods

Cell lines

https://doi.org/10.1016/j.ymeth.2014.01.006Get rights and content
Under a Creative Commons license
open access

Highlights

  • About 150 Drosophila cell lines from a variety of tissues sources exist in a community resource.

  • Extensive genomic and transcriptional data are available for many of these lines.

  • We review basic techniques for culture, transformation, and storage of cell lines.

  • We survey the wide range of applications for which Drosophila lines have been successfully used.

Abstract

We review the properties and uses of cell lines in Drosophila research, emphasizing the variety of lines, the large body of genomic and transcriptional data available for many of the lines, and the variety of ways the lines have been used to provide tools for and insights into the developmental, molecular, and cell biology of Drosophila and mammals.

Abbreviations

DGRC
Drosophila Genomics Resource Center
modENCODE
Model Organism Encyclopedia of DNA Elements

Keywords

Drosophila
Cell lines
Transformation
Transcriptome

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