Elsevier

Mycological Research

Volume 109, Issue 4, April 2005, Pages 429-438
Mycological Research

Ampelomyces mycoparasites from apple powdery mildew identified as a distinct group based on single-stranded conformation polymorphism analysis of the rDNA ITS region

https://doi.org/10.1017/S0953756204001820Get rights and content

Pycnidial fungi belonging to the genus Ampelomyces are the most common natural antagonists of powdery mildews worldwide. During a study of the interactions between apple powdery mildew (Podosphaera leucotricha) and Ampelomyces mycoparasites, 52 new Ampelomyces isolates were obtained from P. leucotricha and, in addition, 13 new isolates from other species of the Erysiphaceae in four European countries. Their genetic diversity was screened using single-stranded conformation polymorphism (SSCP) analysis of the internal transcribed spacer (ITS) region of the ribosomal DNA (rDNA). For comparison, 24 isolates obtained from genetic resource collections or other sources were included in this study. Based on the ITS-SSCP patterns, the isolates were placed in eight groups. The isolates belonged to two types based on their growth in culture. The faster-growing and the slower-growing isolates were included in different SSCP groups. A phylogenetic analysis of the ITS sequences of representatives of these groups confirmed the results obtained with the SSCP method, and showed that the faster-growing isolates do not belong to Ampelomyces as suggested by earlier studies. All the isolates from P. leucotricha fell into a distinct SSCP group of genetically homogeneous isolates. This suggests that Ampelomyces mycoparasites which occur in apple powdery mildew are slightly different from the other Ampelomyces groups which contain mycoparasites from various powdery mildew species. This may be because the main growth period of Ampelomyces mycoparasites in apple powdery mildew is isolated in time from that of Ampelomyces isolates that occur in other species of the Erysiphaceae. P. leucotricha starts its life-cycle early in the season, usually in March-April, while most powdery mildews are active in the same environments only late in the year.

References (43)

  • S.P. Falk et al.

    Partial control of grape powdery mildew by the mycoparasite Ampelomyces quisqualis

    Plant Disease

    (1995)
  • D.A. Filatov

    ProSeq: A software for preparation and evolutionary analysis of DNA sequence data sets

    Molecular Ecology Notes

    (2002)
  • M. Gardes et al.

    ITS primers with enhanced specificity for basidiomycetes-application to the identification of mycorrhizae and rusts

    Molecular Ecology

    (1993)
  • D. Griffith

    The common parasite of the powdery mildews

    Bulletin of the Torrey Botanical Club

    (1899)
  • Y. Hashioka et al.

    Ultrastructure of pycnidial development and mycoparasitism of Ampelomyces quisqualis parasitic on Erysiphales

    Transactions of the Mycological Society of Japan

    (1980)
  • W.R. Jarvis et al.

    The control of powdery mildew of greenhouse cucumber by water spray and Ampelomyces quisqualis

    Plant Disease Reporter

    (1977)
  • W.R. Jarvis et al.

    Epidemiology of powdery mildews in agricultural pathosystems.

  • L. Kiss

    Natural occurrence of Ampelomyces intracellular mycoparasites in mycelia of powdery mildew fungi

    New Phytologist

    (1998)
  • L. Kiss

    Fungal antagonists of powdery mildews and their potential as biocontrol agents

    Pest Management Science

    (2003)
  • L. Kiss et al.

    Ribosomal DNA internal transcribed spacer sequences do not support the species status of Ampelomyces guisqualis, a hyperparasite of powdery mildew fungi

    Current Genetics

    (1998)
  • L. Kiss et al.

    New approaches in the study of the genus Ampelomyces, hyperparasites of powdery mildew fungi.

  • Cited by (44)

    • Ampelomyces

      2020, Beneficial Microbes in Agro-Ecology: Bacteria and Fungi
    • Management of peach rusty spot epidemics with biorational fungicides

      2013, Crop Protection
      Citation Excerpt :

      The specificity of mycoparasites may be another possible cause for lack of peach rusty spot control. In a recent study, Ampelomyces isolates were obtained from P. leucotricha and compared to Ampelomyces mycoparasites from other species of the Erysiphaceae (Szentiványia et al., 2005). Results of the molecular-based analyses indicated that the Ampelomyces mycoparasites from apple powdery mildew belong to a distinct genetically homogenous group that was different from other Ampelomyces groups consisting of mycoparasites from other powdery mildew species.

    View all citing articles on Scopus

    Present address: Eötvös Loránd University, Department of Plant Anatomy, H-1117 Budapest, Pázmány Péter sétány 1/C, Hungary.

    View full text