Photosynthetica 1997, 33(12):265-279 | DOI: 10.1023/A:1006800909651

Rapid and straightforward estimates of photosynthetic characteristics using a portable gas exchange system

R. Parsons1, J.D.B. Weyers1, T. Lawson1, I.M. Godber1
1 Department of Biological Sciences, University of Dundee, Dundee, U.K

Procedures are described for estimating photosynthetic characteristics using a portable infra-red gas analysis (IRGA) system. Once the effects of stomatal limitation on CO2 assimilation have been established, up to ten parameters of photosynthesis can be estimated for a single leaf within 2 h, including: photosynthetic efficiency and capacity on both photon and CO2 bases; compensation irradiances and CO2 compensation concentrations; and light and dark respiration rates. These measurements can be made in the laboratory, glasshouse or field with relative ease. Methods for obtaining near instantaneous ("snapshot") measurements of leaf photosynthesis are also described, using carefully pre-set conditions within the leaf cuvette. Representative results are shown for Phaseolus vulgaris L. Important aspects of the procedure's experimental design, assumptions made in the analysis, and limitations of this approach are analysed.

Prepublished online: January 1, 1998; Published: March 1, 1997  Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Parsons, R., Weyers, J.D.B., Lawson, T., & Godber, I.M. (1997). Rapid and straightforward estimates of photosynthetic characteristics using a portable gas exchange system. Photosynthetica34(2), 265-279. doi: 10.1023/A:1006800909651
Download citation

References

  1. Brooks, A., Farquhar, G.D.: Effect of temperature on the CO2/O2 specificity of ribulose-1,5-bisphosphate carboxylase/oxygenase and the rate of respiration in the light. Estimates from gas-exchange measurements on spinach.-Planta 165: 397-406, 1985. Go to original source...
  2. Caemmerer, S. von, Farquhar, G.D.: Some relationships between the biochemistry of photosynthesis and the gas exchange of leaves.-Planta 153: 376-387, 1981. Go to original source...
  3. Cheeseman, J.M.: PATCHY: Simulating and visualizing the effects of stomatal patchiness on photosynthetic CO2 exchange studies.-Plant Cell Environ. 14: 593-599, 1991. Go to original source...
  4. Downton, W.J.S., Loveys, B.R., Grant, W.I.R.: Stomatal closure fully accounts for the inhibition of photosynthesis by abscisic acid.-New Phytol. 108: 263-266, 1988. Go to original source...
  5. Farquhar, G.D., Caemmerer, S. von: Modelling of photosynthetic response to environmental conditions.-In: Lange, O.L., Nobel, P.S., Osmond, C.B., Ziegler, H. (ed.). Physiological Plant Ecology II. Pp. 549-587. Springer-Verlag, Berlin-Heidelberg-New York 1982. Go to original source...
  6. Farquhar, G.D., Caemmerer, S. von, Berry, J.A.: A biochemical model of photosynthetic CO2 assimilation in leaves of C3 species.-Planta 149: 78-90, 1980. Go to original source...
  7. Farquhar, G.D., Sharkey, T.D.: Stomatal conductance and photosynthesis.-Annu. Rev. Plant Physiol. 33: 317-345, 1982. Go to original source...
  8. Hall, D.O., Scurlock, J.M.O., Bolhar-Nordenkampf, H.R., Leegood, R.C., Long, S.P. (ed.). Photosynthesis and Production in a Changing Environment - A Field and Laboratory Manual.-Chapman and Hall, London-Glasgow-New York-Tokyo-Melbourne-Madras 1993. Go to original source...
  9. Harley, P.C., Sharkey, T.D.: An improved model of C3 photosynthesis at high CO2: Reversed O2 sensitivity explained by lack of glycerate reentry into the chloroplast.-Photosynth. Res. 27: 169-178, 1991. Go to original source...
  10. Harley, P.C., Thomas, R.B., Reynolds, J.F., Strain, B.R.: Modelling photosynthesis of cotton grown in elevated CO2.-Plant Cell Environ. 15: 271-282, 1992. Go to original source...
  11. Jones, H.G.: Plants and Microclimate: A Quantative Approach to Environmental Plant Physiology.-Cambridge University Press, Cambridge 1992.
  12. Jordan, D.B., Ogren, W.L.: The CO2/O2 specificity of ribulose 1,5-bisphosphate carboxylase/oxygenase. Dependence on ribulosebisphosphate concentration, pH, and temperature.-Planta 161: 308-313, 1984. Go to original source...
  13. Laisk, A.: Calculation of leaf photosynthetic parameters considering the statistical distribution of stomatal apertures.-J. exp. Bot. 34: 1627-1635, 1983. Go to original source...
  14. Laisk, A., Sumberg, A.: Partitioning of the leaf CO2 exchange into components using CO2 exchange and fluorescence measurements.-Plant Physiol. 106: 689-695, 1994. Go to original source...
  15. Lawlor, D.W.: Photosynthesis: Molecular, Physiological and Environmental Processes.-Longman Group, Harlow 1993.
  16. Leegood, R.C.: Effects of temperature on photosynthesis and photorespiration.-In: Smernoff, N. (ed.): Environment and Plant Metabolism. Flexibility and Acclimation. Pp. 45-62. Bios Scientific Publishers, Oxford 1995.
  17. Long, S.P., Hällgren, J.-E.: Measurement of CO2 assimilation by plants in the field and the laboratory.-In: Hall, D.O., Scurlock, J.M.O., Bolhàr-Nordenkampf, H.R., Leegood, R.C., Long, S.P. (ed.): Photosynthesis and Production in a Changing Environment - A Field and Laboratory Manual. Pp. 129-167. Chapman and Hall, London-Glasgow-New York-Tokyo-Melbourne-Madras 1993. Go to original source...
  18. Mansfield, T.A., Hetherington, A.M., Atkinson, C.J.: Some current aspects of stomatal physiology.-Annu. Rev. Plant Physiol. Plant mol. Biol. 41: 55-75, 1990. Go to original source...
  19. Parsons, R., Sprent, J.I., Raven, J.A.: A simple open flow system used to measure acetylene reduction activity of Sesbania rostrata stem and root nodules.-J. exp. Bot. 43: 595-604, 1992. Go to original source...
  20. Parkinson, K.J., Day, W., Leach, J.E.: A portable system for measuring the photosynthesis and transpiration of graminaceous leaves.-J. exp. Bot. 31: 1441-1453, 1990. Go to original source...
  21. Pearcy, R.W., Ehleringer, J., Mooney, H.A., Rundel, P.W.: Plant Physiological Ecology. Field Methods and Instrumentation.-Chapman and Hall, London 1991.
  22. Pettersson, R., McDonald, A.J.S.: Effects of nitrogen supply on the acclimation of photosynthesis to elevated CO2.-Photosynth. Res. 39: 389-400, 1994. Go to original source...
  23. Sharkey, T.D.: Photosynthesis in intact leaves of C3 plants: physics, physiology and rate limitations.-Bot. Rev. 51: 53-105, 1985. Go to original source...
  24. Terashima, I., Sakaguchi, S., Hara, N.: Intra-leaf and intracellular gradients in chloroplast ultrastructure of dorsiventral leaves illuminated from the adaxial or abaxial side during their development.-Plant Cell Physiol. 27: 1023-1031, 1986.
  25. Weyers, J.D.B., Lawson, T., Peng, Z.Y.: Variation in stomatal characters at the whole leaf level.-In: van Gardingen, P.R., Foody, G.M., Curren, P.J. (ed.): Scaling Up. In Press, Cambridge University Press, Cambridge 1997.
  26. Weyers, J.D.B., Meidner, H.: Methods in Stomatal Research.-Longman Group, Harlow 1990.
  27. Wullschleger, S.D.: Biochemical limitations to carbon assimilation in C3 plants - A retrospective analysis of the A/C i curves from 109 species.-J. exp. Bot. 44: 907-920, 1993. Go to original source...
  28. Zelitch, I.: Photorespiration: Studies with whole tissues.-In: Gibbs, M., Latzko, E. (ed.): Photosynthesis II. Pp. 353-367. Springer-Verlag, Berlin-Heidelberg-New York 1979. Go to original source...