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Temporal and spatial control of cyclin B1 destruction in metaphase

Nature Cell Biology volume 1, pages 82–87 (1999)Cite this article

Abstract

The proteolysis of key regulatory proteins is thought to control progress through mitosis. Here we analyse cyclin B1 degradation in real time and find that it begins as soon as the last chromosome aligns on the metaphase plate, just after the spindle-assembly checkpoint is inactivated. At this point, cyclin B1 staining disappears from the spindle poles and from the chromosomes. Cyclin B1 destruction can subsequently be inactivated throughout metaphase if the spindle checkpoint is reimposed, and this correlates with the reappearance of cyclin B1 on the spindle poles and the chromosomes. These results provide a temporal and spatial link between the spindle-assembly checkpoint and ubiquitin-mediated proteolysis.

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Figure 1: Real time visualization and measurement of cyclin B1 proteolysis.
Figure 2: Cyclin B1 destruction begins when chromosomes align on the metaphase plate.
Figure 3: Taxol can turn off cyclin B1 destruction once it has started.
Figure 4: The redistribution of cyclin B1–GFP is not directly due to proteolysis.

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Acknowledgements

We thank I. Hagan for suggesting the taxol experiment and for discussions, S. Geley and T. Hunt for the Xenopus CSF extract, J. Huang and J. Raff for unpublished observations, and M. Zernicka-Goetz, T. Kouzarides, and the members of our laboratory for support and discussions. We also thank W. Earnshaw and C. Rieder for their enthusiasm and support. P.C. was an 1851 Exhibition fellow and is supported by the Association for International Cancer Research. This work was supported by a programme grant from the Cancer Research Campaign and an MRC project grant.

Correspondence and requests for materials should be addressed to J.P.

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    Paul Clute & Jonathon Pines

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Correspondence to Jonathon Pines.

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Clute, P., Pines, J. Temporal and spatial control of cyclin B1 destruction in metaphase. Nat Cell Biol 1, 82–87 (1999). https://doi.org/10.1038/10049

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