Abstract
T7 mutant in gene 4 synthesizes only short DNA fragments complementary to the H strand. By an in vitro complementation system which starts from intact T7 DNA, gene 4 protein has been purified.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Grippo, P., and Richardson, C. C., J. biol. Chem., 246, 6867 (1971).
Oey, J. L., Strätling, W., and Knippers, R., Eur. J. Biochem., 23, 497 (1971).
Masamune, Y., Frenkel, G. D., and Richardson, C. C., J. biol. Chem., 246, 6874 (1971).
Strätling, W., Ferdinand, F.-J., and Knippers, R. (in the press).
Hausmann, R., and Gomez, B., J. Virol., 1, 779 (1967).
Hausmann, R., and LaRue, K., Virology, 3, 278 (1969).
Studier, F. W., Virology, 39, 562 (1969).
Strätling, W., Krause, E., and Knippers, R., Virology, 51, 109 (1973).
Center, M. S., Studier, F. W., and Richardson, C. C., Proc. natn. Acad. Sci. U.S.A., 65, 242 (1970).
Studier, F. W., J. molec. Biol., 11, 373 (1965).
Knippers, R., Strätling, W., and Krause, E., in DNA Synthesis In Vitro (edit. by Wells, R. D., and Inman, R. B.) (University Park Press, Baltimore, 1973).
Summers, W. C., and Szybalski, W., Virology, 34, 9 (1968).
Gillespie, D., and Spiegelman, S., J. molec. Biol., 12, 829 (1965).
Denhardt, D. T., Biochem. biophys. Res. Commun., 23, 641 (1966).
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J., J. biol. Chem., 193, 265 (1951).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
STRÄTLING, W., KNIPPERS, R. Function and Purification of Gene 4 Protein of Phage T7. Nature 245, 195–197 (1973). https://doi.org/10.1038/245195a0
Received:
Issue Date:
DOI: https://doi.org/10.1038/245195a0
This article is cited by
-
Replication of the colicin E1 plasmid in extracts of Escherichia coli: Uncoupling of leading strand from lagging strand synthesis
Molecular and General Genetics MGG (1979)
-
Studies on bacteriophage T7 DNA synthesis in vitro
Molecular and General Genetics MGG (1975)
-
Some functions involved in bacteriophage T7 genetic recombination
Molecular and General Genetics MGG (1974)
-
In vitro studies on the role of phage T7 gene 4 product in DNA replication
Molecular and General Genetics MGG (1974)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.