Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Function and Purification of Gene 4 Protein of Phage T7

Abstract

T7 mutant in gene 4 synthesizes only short DNA fragments complementary to the H strand. By an in vitro complementation system which starts from intact T7 DNA, gene 4 protein has been purified.

This is a preview of subscription content, access via your institution

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Grippo, P., and Richardson, C. C., J. biol. Chem., 246, 6867 (1971).

    CAS  PubMed  Google Scholar 

  2. Oey, J. L., Strätling, W., and Knippers, R., Eur. J. Biochem., 23, 497 (1971).

    Article  CAS  Google Scholar 

  3. Masamune, Y., Frenkel, G. D., and Richardson, C. C., J. biol. Chem., 246, 6874 (1971).

    CAS  Google Scholar 

  4. Strätling, W., Ferdinand, F.-J., and Knippers, R. (in the press).

  5. Hausmann, R., and Gomez, B., J. Virol., 1, 779 (1967).

    CAS  PubMed  PubMed Central  Google Scholar 

  6. Hausmann, R., and LaRue, K., Virology, 3, 278 (1969).

    CAS  Google Scholar 

  7. Studier, F. W., Virology, 39, 562 (1969).

    Article  CAS  Google Scholar 

  8. Strätling, W., Krause, E., and Knippers, R., Virology, 51, 109 (1973).

    Article  Google Scholar 

  9. Center, M. S., Studier, F. W., and Richardson, C. C., Proc. natn. Acad. Sci. U.S.A., 65, 242 (1970).

    Article  ADS  CAS  Google Scholar 

  10. Studier, F. W., J. molec. Biol., 11, 373 (1965).

    Article  CAS  Google Scholar 

  11. Knippers, R., Strätling, W., and Krause, E., in DNA Synthesis In Vitro (edit. by Wells, R. D., and Inman, R. B.) (University Park Press, Baltimore, 1973).

    Google Scholar 

  12. Summers, W. C., and Szybalski, W., Virology, 34, 9 (1968).

    Article  CAS  Google Scholar 

  13. Gillespie, D., and Spiegelman, S., J. molec. Biol., 12, 829 (1965).

    Article  CAS  Google Scholar 

  14. Denhardt, D. T., Biochem. biophys. Res. Commun., 23, 641 (1966).

    Article  CAS  Google Scholar 

  15. Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J., J. biol. Chem., 193, 265 (1951).

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

STRÄTLING, W., KNIPPERS, R. Function and Purification of Gene 4 Protein of Phage T7. Nature 245, 195–197 (1973). https://doi.org/10.1038/245195a0

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1038/245195a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing