Abstract
Fluorescein isothiocyanate-labelled integral membrane proteins are mobile in the membranes of human erythrocytes that have been fused (and haemolysed) by Sendai virus or polyethylene glycol. Minimum diffusion coefficients are of the order of 10−11 cm2 s−1 at 37 °C. This mobility is reduced several-fold at room temperature, not detected at 0 °C, and is significantly greater in fresh than in aged blood. Mobility was assessed by observing the spread of fluorescence on labelled cells which had been fused with unlabelled cells; neither intramembrane particle aggregation nor spectrin release occurred during this process.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Edelman, G. M. Science 192, 218 (1976).
Nicolson, G. L. Biochim. biophys. Acta 457, 57 (1976).
Singer, S. J. A. Rev. Biochem. 43, 805 (1974).
Loor, F., Forni, F. & Pernis, B. Eur. J. Immun. 2, 203 (1972).
Cohen, F., Zuelzer, W. W. & Evans, M. M. Blood 15, 884 (1960).
Chan, L.-N. L. & Oliver, J. M. J. Cell Biol. 69, 647 (1976).
Pinto da Silva, P. J. Cell Biol. 53, 777 (1972).
Elgsaeter, A. & Branton, D. J. Cell Biol. 63, 1018 (1974).
Branton, D. & Deamer, D. W. Protoplasmatologia 2, E, (Springer, New York, 1972).
Yu, J. & Branton, D. Proc. natn. Acad. Sci. U.S.A. 73, 3891 (1976).
Pinto da Silva, P., Douglas, S. D. & Branton, D. Nature 232, 194 (1971).
Marchesi, V. T., Tillack, T. W., Jackson, R. L., Segrest, J. P. & Scott, R. E. Proc. natn. Acad. Sci. U.S.A. 69, 1445 (1972).
Pinto da Silva, P. & Nicolson, G. L. Biochim. biophys. Acta 363, 311 (1974).
Tillack, T. W., Scott, R. E. & Marchesi, V. J. J. exp. Med. 135, 1209 (1972).
Peters, R., Peters, J., Tews, K. H. & Bahr, W. Biochim. biophys. Acta 367, 282 (1974).
Kury, P. G., Ramwell, P. W. & McConnell, H. M. Biochem. biophys. Res. Commun. 56, 178 (1974).
Kury, P. G. & McConnell, H. M. Biochemistry 14, 2798 (1975).
Meyers, M. B. & Swislocki, N. I. Archs Biochem. Biophys. 164, 544 (1974).
Allen, J. E. & Rasmussen, H. Science 174, 512 (1971).
Gordon, J. A. & Marquardt, M. D. Nature 258, 346 (1975).
Bächi, T. & Schnebli, H. P. Expl Cell Res. 91, 285 (1975).
Frye, L. D. & Edidin, M. J. Cell Sci. 7, 319 (1970).
Singer, S. F. & Nicolson, G. L. Science 175, 720 (1972).
Steck, T. L. J. Cell Biol. 62, 1 (1974).
Dodge, J. T., Mitchell, C. & Hanahan, D. J. Archs Biochem. Biophys. 100, 119 (1963).
Fairbanks, G., Steck, T. L. & Wallach, D. F. H. Biochemistry 10, 2606 (1971).
Bretscher, M. S. Nature new. Biol. 231, 229 (1971).
Davidson, R. L. & Gerald, P. S. Somatic Cell Genetics 2, 165 (1976).
Steck, T. L. & Yu, J. J. supramolec. Struct. 1, 220 (1973).
Avruch, J., Price, H. D., Martin, D. B. & Carter, J. M. Biochim. biophys. Acta 291, 494 (1973).
Steck, R. L., Fairbanks, G. & Wallach, D. F. H. Biochemistry 10, 2617 (1971).
Huang, H. W. J. theor. Biol. 40, 11 (1973).
Edidin, M., Zagyansky, Y. & Lardner, T. J. Science 191, 466 (1976).
Jacobson, K., Wu, E. & Poste, G. Biochim. biophys. Acta 433, 215 (1976).
Schlessinger, J. et al. Proc. natn. Acad. Sci. U.S.A. 73, 2409 (1976).
Schlessinger, J., Axelrod, D., Koppel, D. E., Webb, W. W. & Elson, E. L. Science 195, 307 (1977).
Edidin, M. A. Rev. Biophys. Bioengng 3, 179 (1974).
Cherry, R. J. Biological Membranes 3, (eds Chapman, D. & Wallach, D. F. H.) 47 (Academic, London and New York, 1976).
Petit, V. A. & Edidin, M. Science 184, 1183 (1974).
Nakao, M., Nakao, T. & Yamazoe, S. Nature 187, 945 (1960).
Weed, R. I., LaCelle, P. L. & Merrill, E. W. J. Clin. Invest. 48, 795 (1969).
Elgsaeter, A., Shotton, D. M. & Branton, D. Biochim. biophys. Acta 426, 101 (1976).
Cherry, R. J., Bürkli, A., Busslinger, M., Schneider, G. & Parrish, G. R. Nature 263, 389 (1976).
Bennett, V. & Büanton, D. J. biol. Chem. 252, 2753 (1977).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Fowler, V., Branton, D. Lateral mobility of human erythrocyte integral membrane proteins. Nature 268, 23–26 (1977). https://doi.org/10.1038/268023a0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/268023a0
This article is cited by
-
Functional Links between Membrane Transport and the Spectrin Cytoskeleton
Journal of Membrane Biology (2006)
-
Direct covalent linkage of fluorescent probes to the plant protoplast surface
Protoplasma (1987)
-
Kinetics of ultrastructural changes during electrically induced fusion of human erythrocytes
The Journal of Membrane Biology (1986)
-
Oligomeric structure and the anion transport function of human erythrocyte band 3 protein
The Journal of Membrane Biology (1984)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.