Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

In vivo sequence requirements of the SV40 early promoter region

Nature volume 290, pages 304–310 (1981)Cite this article

Abstract

To investigate the sequences necessary for proper initiation of transcription of SV40 early genes, we have constructed several deletion mutants in the promoter region. The TATA box region is apparently involved infixing initiation precisely within a narrow area, but is dispensable for gene expression, while the sequences located more than 150 base pairs upstream are indispensable.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Breathnach, R. & Chambon, P. A. Rev. Biochem. (in the press).

  2. Corden, J. et al. Science 209, 1406–1414 (1980).

    Article  ADS  CAS  PubMed  Google Scholar 

  3. Ziff, E. & Evans, R. Cell 15, 1463–1475 (1978).

    Article  CAS  PubMed  Google Scholar 

  4. Flavell, A., Cowie, A., Arrand, J. & Kamen, R. J. Virol. 33, 902–908 (1980).

    CAS  PubMed  PubMed Central  Google Scholar 

  5. Haegeman, G. & Fliers, W. Nucleic Acids Res. 5, 2359–2371 (1978).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Baker, C., Herisse, J., Courtois, G., Galibert, F. & Ziff, E. Cell 18, 569–580 (1979).

    Article  CAS  PubMed  Google Scholar 

  7. Groner, Y., Carmi, P. & Aloni, Y. Nucleic Acids Res. 4, 3959–3968 (1978).

    Article  Google Scholar 

  8. Ghosh, P., Reddy, V., Swinscoe, J., Lebowitz, P. & Weissman, S. J. molec. Biol. 126, 813–846 (1979).

    Article  Google Scholar 

  9. Weil, P., Luse, D., Segall, J. & Roeder, R. Cell 18, 469–484 (1979).

    Article  CAS  PubMed  Google Scholar 

  10. Manley, J., Fire, A., Cano, A., Sharp, P. & Gefter, M. Proc. natn. Acad. Sci. U.S.A. 77, 3855–3859 (1980).

    Article  ADS  CAS  Google Scholar 

  11. Wasylyk, B., Kedinger, C., Corden, J., Brison, O. & Chambon, P. Nature 285, 367–373 (1980).

    Article  ADS  CAS  PubMed  Google Scholar 

  12. Wasylyk, B. et al. Proc. natn. Acad. Sci. U.S.A. (in the press).

  13. Benoist, C. & Chambon, P. Proc. natn. Acad. Sci. U.S.A. 77, 3865–3869 (1980).

    Article  ADS  CAS  Google Scholar 

  14. Gluzman, Y., Sambrook, J. & Frisque, R. Proc. natn. Acad. Sci. U.S.A. 77, 3898–3902 (1980).

    Article  ADS  CAS  Google Scholar 

  15. Mueller, C., Graessmann, A. & Graessmann, M. Cell 15, 579–586 (1978).

    Article  CAS  PubMed  Google Scholar 

  16. Grosschedl, R. & Birnstiel, M. Proc. natn. Acad. Sci. U.S.A. 77, 1432–1436 (1980).

    Article  ADS  CAS  Google Scholar 

  17. Acheson, N. in Molecular Biology of Tumor Viruses Pt 2 (ed. Tooze, J.) 125–204 (Cold Spring Harbor, New York, 1980).

    Google Scholar 

  18. Thompson, J., Radonovich, M. & Salzman, N. J. Virol. 31, 437–446 (1979).

    CAS  PubMed  PubMed Central  Google Scholar 

  19. Reddy, V., Ghosh, P., Lebowitz, P., Piatak, M. & Weissman, S. J. Virol. 30, 279–296 (1979).

    CAS  PubMed  PubMed Central  Google Scholar 

  20. Haegeman, G. & Fiers, W. J. Virol. 35, 955–961 (1980).

    CAS  PubMed  PubMed Central  Google Scholar 

  21. Maxam, A. & Gilbert, W. Meth. Enzym. 65, 499–580 (1980).

    Article  CAS  PubMed  Google Scholar 

  22. Alwine, J., Kemp, D. & Stark, G. Proc. natn. Acad. Sci. U.S.A. 74, 5350–5354 (1977).

    Article  ADS  CAS  Google Scholar 

  23. Weaver, R. & Weissmann, C. Nucleic Acids Res. 7, 1175–1193 (1979).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Mathis, D. J. & Chambon, P. Nature 290, 310–315 (1981).

    Article  ADS  CAS  PubMed  Google Scholar 

  25. Reed, S., Stark, G. & Alwine, J. Proc. natn. Acad. Sci. U.S.A. 73, 3083–3087 (1976).

    Article  ADS  CAS  Google Scholar 

  26. Tjian, R. Cell 13, 165–179 (1978).

    Article  CAS  PubMed  Google Scholar 

  27. Shalloway, D., Kleinberger, T. & Livingston, D. Cell 20, 411–422 (1980).

    Article  CAS  PubMed  Google Scholar 

  28. Myers, R. and Tjian, R. Proc. natn. Acad. Sci. U.S.A. 77, 6491–6495 (1980).

    Article  ADS  CAS  Google Scholar 

  29. Van Heuverswyn, H. & Fiers, W. Eur. J. Biochem. 100, 51–60 (1979).

    Article  CAS  PubMed  Google Scholar 

  30. Scott, W. & Wigmore, D. Cell 15, 1511–1518 (1978).

    Article  CAS  PubMed  Google Scholar 

  31. Sundin, O. & Varshavsky, A. J. molec. Biol. 132, 535–546 (1979).

    Article  CAS  PubMed  Google Scholar 

  32. Varshavsky, A., Sundin, O. & Bohn, M. Cell 16, 453–466 (1979).

    Article  CAS  PubMed  Google Scholar 

  33. Saragosti, M., Moyne, G. & Yaniv, M. Cell 20, 65–73 (1980).

    Article  CAS  PubMed  Google Scholar 

  34. Wigmore, D., Eaton, R. & Scott, W. Virology 104, 462–473 (1980).

    Article  CAS  PubMed  Google Scholar 

  35. Stalder, J. et al. Cell 20, 451–460 (1980).

    Article  CAS  PubMed  Google Scholar 

  36. Wu, C. Nature 286, 854–860 (1980).

    Article  ADS  CAS  PubMed  Google Scholar 

  37. Flavell, R. Nature 285, 356–357 (1980).

    Article  ADS  CAS  PubMed  Google Scholar 

  38. Mathis, D., Oudet, P. & Chambon, P. Prog. Nucleic Acids Res. molec. Biol. 24, 1–55 (1980).

    Article  CAS  Google Scholar 

  39. Mertz, J. & Berg, P. Virology 62, 112–124 (1974).

    Article  CAS  PubMed  Google Scholar 

  40. Wigler, M., Pellicer, A., Silverstein, S. & Axel, R. Cell 14, 725–731 (1978).

    Article  CAS  PubMed  Google Scholar 

  41. Reddy, V. et al. Science 200, 494–502 (1978).

    Article  ADS  CAS  PubMed  Google Scholar 

  42. Legerski, R., Hodnett, J. & Gray, H. Nucleic Acids Res. 5, 1445–1464 (1978).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  43. Fiers, W. et al. Nature 273, 113–120 (1978).

    Article  ADS  CAS  PubMed  Google Scholar 

  44. Sanger, F. & Coulson, A. FEBS Lett. 87, 107–110 (1977).

    Google Scholar 

  45. Favaloro, J., Treisman, R. & Kamen, R. Meth. Enzym. 65, 718–749 (1980).

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Author notes

  1. Christophe Benoist

    Present address: Department of Genetics, Stanford University Medical School, Stanford, California, 94305, USA

Authors and Affiliations

  1. Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Unité 184 de Biologie Moléculaire et de Génie Génétique de l'INSERM, Institut de Chimie Biologique, Faculté de Médecine, 67085, Strasbourg, France

    Christophe Benoist & Pierre Chambon

Authors
  1. Christophe Benoist
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Pierre Chambon
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Benoist, C., Chambon, P. In vivo sequence requirements of the SV40 early promoter region. Nature 290, 304–310 (1981). https://doi.org/10.1038/290304a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/290304a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing