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Structure of the S-layer of Sulfolobus acidocaldarius

Abstract

The outermost component of many bacterial cell envelopes is a two-dimensional crystalline array of protein molecules, termed the S-layer1. Despite considerable effort to investigate its structure and composition, its precise function remains uncertain. We report here the first determination of the three-dimensional structure of an S-layer, that from the thermophilic bacterium, Sulfolobus acidocaldarius. Our map shows a complex and strongly interconnected structure, penetrated by numerous channels which appear to provide little barrier to anything but rather large molecules. The external surface is fairly smooth, but the cellular side is sculpted with large cavities and protruding ‘pedestals’. The protein substructure consists of three types of globular domain, connected by narrow bridges. We suggest that these may be flexible ‘hinges’, allowing the S-layer to form a curved surface, and that the multidomain structure allows the S-layer to maintain strong connectivity as it grows with the bacterium.

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References

  1. Sleytr, U. B. Int. Rev. Cytol. 53, 1–64 (1978).

    Article  CAS  Google Scholar 

  2. Brock, T. D. Thermophilic Microorganisms and Life at High Temperatures, 117–179 (Springer, New York, 1978).

    Google Scholar 

  3. Weiss, R. L. J. gen. Microbiol. 77, 501–507 (1973).

    Article  CAS  Google Scholar 

  4. Weiss, R. L. J. Bact. 118, 275–284 (1974).

    CAS  PubMed  Google Scholar 

  5. Michel, H., Neugebauer, D.-Ch. & Oesterhelt, D. in Electron Microscopy at Molecular Dimensions (eds Baumeister, W. & Vogell, W.) 27–35 (Springer, New York, 1980).

    Book  Google Scholar 

  6. Brock, T. D., Brock, K. M., Belly, R. T. & Weiss, R. L. Arch. Mikrobiol. 84, 54–68 (1972).

    Article  CAS  Google Scholar 

  7. Harrison, S. C., Olson, A. J., Schutt, C. E., Winkler, F. K. & Bricogne, G. Nature 276, 368–373 (1978).

    Article  ADS  CAS  Google Scholar 

  8. Harris, W. F. & Scriven, L. E. Nature 228, 827–829 (1970).

    Article  ADS  CAS  Google Scholar 

  9. Watson, S. W. & Remsen, C. C. Science 163, 685 (1969).

    Article  ADS  CAS  Google Scholar 

  10. Sleytr, U. B. & Glauert, A. M. J. ultrastruct. Res. 50, 103–116 (1975).

    Article  Google Scholar 

  11. Caspar, D. L. D. & Klug, A. Cold Spring Harb. Symp. quant. Biol. 27, 1–24 (1962).

    Article  CAS  Google Scholar 

  12. Heuser, J. J. Cell Biol. 84, 560–583 (1980).

    Article  CAS  Google Scholar 

  13. Glaeser, R. M., Chiu, W. & Grano, D. J. ultrastruct. Res. 66, 235–242 (1979).

    Article  CAS  Google Scholar 

  14. Thorne, K. J. I. Biol. Rev. 52, 219–234 (1977).

    Article  CAS  Google Scholar 

  15. Cohen-Bazire, G., Kunisawa, R. & Pfennig, N. J. Bact. 100, 1049–1061 (1969).

    CAS  Google Scholar 

  16. Wilkinson, J. F. Symp. Soc. gen. Microbiol. 21, 15–46 (1971).

    CAS  Google Scholar 

  17. Stewart, M. & Beveridge, T. J. J. Bact. 142, 302–309 (1980).

    CAS  PubMed  Google Scholar 

  18. Stewart, M., Beveridge, T. J. & Murray, R. G. E. J. molec. Biol. 137, 1–8 (1980).

    Article  CAS  Google Scholar 

  19. Henderson, R. & Unwin, P. N. T. Nature 257, 28–32 (1975).

    Article  ADS  CAS  Google Scholar 

  20. Deatherage, J. F., Taylor, K. A. & Amos, L. A. J. molec. Biol. (submitted).

Download references

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Taylor, K., Deatherage, J. & Amos, L. Structure of the S-layer of Sulfolobus acidocaldarius. Nature 299, 840–842 (1982). https://doi.org/10.1038/299840a0

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