Abstract
The sequence of a 4,869 base–pair fragment of Drosophila melanogaster mitochondrial DNA is presented. It contains genes for cytochrome oxidase subunits I, II and III, ATPase subunit 6 and six tRNAs together with two unassigned reading frames. The gene organization differs from that of mammalian mitochondrial DNAs. Evidence is provided for a genetic code in which AGA codes for serine and the quadruplet ATAA is used in initiation of translation.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Fauron, C. M.-R. & Wolstenholme, D. R. Proc. natn. Acad. Sci. U.S.A. 73, 3623–3627 (1976).
Peacock, W. J. et al. Cold Spring Harb. Symp. quant. Biol. 38, 405–416 (1973).
Shah, D. M. & Langley, C. H. Nature 281, 696–699 (1979).
Fauron, C. M.-R. & Wolstenholme, D. R. Nucleic Acids Res. 8, 2439–2452 (1980).
Goddard, J. M. & Wolstenholme, D. R. Nucleic Acids Res. 8, 741–757 (1980).
Klukas, C. K. & Dawid, I. B. Cell 9, 615–625 (1976).
Anderson, S. et al. Nature 290, 457–465 (1981).
Anderson, S. et al. J. molec. Biol. 156, 683–717 (1982).
Bibb, M. J., Van Etten, R. A., Wright, C. T., Walberg, M. W. & Clayton, D. A. Cell 26, 167–180 (1981).
Ramirez, J. L. & Dawid, I. B. J. molec. Biol. 119, 133–146 (1978).
Spradling, A., Pardue, M. L. & Penman, S. J. molec. Biol. 109, 559–587 (1977).
Merten, S. H. & Pardue, M. L. J. molec. Biol. 153, 1–21 (1981).
Clary, D. O., Goddard, J. M., Martin, S. C., Fauron, C. M.-R. & Wolstenholme, D. R. Nucleic Acids Res. 10, 6619–6637 (1982).
Staden, R. Nucleic Acids Res. 8, 817–825 (1980).
Barrell, B. G., Bankier, A. T. & Drouin, J. Nature 282, 189–194 (1979).
Martens, P. A. & Clayton, D. A. J. molec. Biol. 135, 327–351 (1979).
Young, I. G. & Anderson, S. Gene 12, 257–265 (1980).
Macino, G., Coruzzi, G., Nobrega, F. G., Li, M. & Tzagoloff, A. Proc. natn. Acad. Sci. U.S.A. 76, 3784–3785 (1979).
Yin, S. et al. in Mitochondrial Genes (eds Slonimski, P., Borst, P. & Attardi, G.) 361–373 (Cold Spring Harbor Laboratory, New York, 1982).
Waring, R. B. et al. Cell 27, 4–11 (1981).
Bonitz, S. G. et al. Proc. natn. Acad. Sci. U.S.A. 77, 3167–3170 (1980).
Heckman, J. E., Sarnoff, J., Alzner-DeWeerd, B., Yin, S. & RajBhandary, U. L. Proc. natn. Acad. Sci. U.S.A. 77, 3159–3163 (1980).
Fox, T. D. Proc. natn. Acad. Sci. U.S.A. 76, 6534–6538 (1979).
Bonitz, S. G., Coruzzi, G., Thalenfeld, B. E., Tzagoloff, A. & Macino, G. J. biol. Chem. 255, 11927–11941 (1980).
Thalenfeld, B. E. & Tzagoloff, A. J. biol. Chem. 255, 6173–6180 (1980).
Macino, G. & Tzagoloff, A. Cell 20, 507–517 (1980).
Dickerson, R. E. & Geis, I. The Structure and Action of Proteins, 19–20 (Benjamin/Cummings, Menlo Park, 1969).
Hudspeth, M. E. S., Ainley, M. W., Shumard, D. S., Butow, R. A. & Grossman, L. I. Cell 30, 617–626 (1982).
Mason, P. J. & Bishop, J. O. Biochem. biophys. Res. Commun. 95, 1268–1274 (1980).
Grosskopf, R. & Feldmann, H. Curr. Genet. 4, 151–158 (1981).
Browning, K. S. & RajBhandary, U. L. J. biol. Chem. 257, 5253–5256 (1982).
Netzker, R., Köchel, H. G., Basak, N. & Küntzel, H. Nucleic Acids Res. 10, 4783–4794 (1982).
Fox, T. D. & Leaver, C. J. Cell 26, 315–323 (1981).
Beremand, M. N. & Blumenthal, T. Cell 18, 257–266 (1979).
Donahue, T. F., Farabaugh, P. J. & Fink, G. R. Science 212, 455–457 (1981).
Riddle, D. L. & Roth, J. R. J. molec. Biol. 66, 495–506 (1972).
Riddle, D. L. & Carbon, J. Nature new Biol. 242, 230–234 (1973).
Atkins, J. F. in Transfer RNA: Biological Aspects (eds Söll, D., Abelson, J. N. & Schimmel, P. R.) 439–449 (Cold Spring Harbor Laboratory, New York, 1979).
Atkins, J. F. & Ryce, S. Nature 249, 527–529 (1974).
Bossi, L. & Roth, J. R. Nature 286, 123–127 (1980).
Kurland, C. G. in Nonsense Mutations and tRNA Suppressors (eds Celis, J. E. & Smith, J. D.) 97–108 (Academic, New York, 1979).
Fox, T. D. & Weiss-Brummer, B. Nature 288, 60–63 (1980).
Yourno, J. Nature new Biol. 239, 219–221 (1972).
Bossi, L. & Roth, J. R. Cell 25, 489–496 (1981).
Gaber, R. F. & Culbertson, M. R. Gene 19, 163–172 (1982).
Atkins, J. F., Gesteland, R. F., Reid, B. R. & Anderson, C. W. Cell 18, 1119–1131 (1979).
Macino, G. & Tzagoloff, A. Proc. natn. Acad. Sci. U.S.A. 76, 131–135 (1979).
Sibler, A.-P., Dirheimer, G. & Martin, R. P. FEBS Lett. 132, 344–348 (1981).
Attardi, G. et al. in Proc. ICN-UCLA Symp. Extrachromosomal DNA (eds Cummings, D., Borst, P., Dawid, I. Weissman, S. & Fox, C. F.) 443–469 (Academic, New York, 1979).
Barrell, B. G. et al. in 31st Mosbach Colloq. Biological Chemistry of Organelle Formation (eds Bücher, T. H., Sebald, W. & Weiss, H.) 11–25 (Springer, Berlin, 1980).
Ojala, D., Montoya, J. & Attardi, G. Nature 290, 470–474 (1981).
Ojala, D., Merkel, C., Gelfand, R. & Attardi, G. Cell 22, 393–403 (1980).
Montoya, J., Ojala, D. & Attardi, G. Nature 290, 465–470 (1981).
Borst, P. & Grivell, L. A. Nature 290, 443–444 (1981).
Dirheimer, G., Keith, G., Sibler, A. -P. & Martin, R. P. in Transfer RNA: Structure, Properties and Recognition (eds Schimmel, P. R., Söll, D. & Abelson, J. N.) 19–41 (Cold Spring Harbor Laboratory, New York, 1979).
Eigen, M. & Winkler-Oswatitsch, R. Naturwissenschaften 68, 217–228 (1981).
Dubin, D. T., Montoya, J., Timko, K. D. & Attardi, G. J. molec. Biol. 157, 1–19 (1982).
Köchel, H. G., Lazarus, C. M., Basak, N. & Küntzel, H. Cell 23, 625–633 (1981).
Martin, R. P., Schneller, J.-M., Stahl, A. J. C. & Dirheimer, G. Biochemistry 18, 4600–4605 (1979).
Dayhoff, M. O. Atlas of Protein Sequence and Structure (National Biomedical Research Foundation, Silversprings, Maryland, 1969).
Staden, R. Nucleic Acids Res. 4, 4037–4051 (1977).
Sanger, F., Coulson, A. R., Barrell, B. G., Smith, A. J. H. & Roe, B. A. J. molec. Biol. 143, 161–178 (1980).
Deininger, P. Analyt. Biochem. 129, 216–223 (1983).
Messing, J. & Vieira, J. Gene 19, 269–276 (1982).
Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).
Sanger, F. & Coulson, A. R., FEBS Lett. 87, 107–110 (1978).
Duckworth, M. L. et al. Nucleic Acids Res. 9, 1691–1706 (1981).
Staden, R. Nucleic Acids Res. 10, 4731–4751 (1982).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
de Bruijn, M. Drosophila melanogaster mitochondrial DNA, a novel organization and genetic code. Nature 304, 234–240 (1983). https://doi.org/10.1038/304234a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/304234a0
This article is cited by
-
The first complete mitochondrial genome sequences for Ulidiidae and phylogenetic analysis of Diptera
Molecular Biology Reports (2023)
-
Mitochondrial genome sequencing, mapping, and assembly benchmarking for Culicoides species (Diptera: Ceratopogonidae)
BMC Genomics (2022)
-
The first complete mitochondrial genome of Limnadia lenticularis (Branchiopoda, Spinicaudata), with new insights on its phylogeography and on the taxonomy of the genus
Hydrobiologia (2019)
-
Mitochondrial genomes and comparative analyses of Culex camposi, Culex coronator, Culex usquatus and Culex usquatissimus (Diptera:Culicidae), members of the coronator group
BMC Genomics (2015)
-
Description of new mitochondrial genomes (Spodoptera litura, Noctuoidea and Cnaphalocrocis medinalis, Pyraloidea) and phylogenetic reconstruction of Lepidoptera with the comment on optimization schemes
Molecular Biology Reports (2013)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.