Abstract
Mouse t-haplotypes demonstrate strong linkage disequilibrium between t-lethal genes and specific H–2 types, presumably a result of recombination suppression between t and normal chromosomes1. The observation of free recombination occurring between two complementary t-haplotypes suggested a chromosomal mismatch between t and normal chromosomes2. Recent data showing the H–2 complex to be misplaced relative to two other markers, T and tf, in t-haplotypes3 suggested that chromosomal rearrangement in t-haplotypes might be the basis for their ‘mismatch’ with the normal chromosome. Here, to analyse the molecular nature of the rearrangement, we have cloned a polymorphic H–2 class I restriction fragment, which had previously been shown to map centromeric to the serologically defined H–2 complex in t-haplotypes4. Genetic mapping studies show that this cloned t-DNA is homologous to the H–2 D region of wild-type chromosomes, and that the Eα Ia gene maps telomeric to this DNA fragment in t-haplotypes, in contrast to its orientation in wild-type chromosomes. These results give molecular evidence for an inversion of H–2 in t-haplotypes, which may be at least partially responsible for recombination suppression and thus for linkage disequilibrium.
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Shin, HS., Flaherty, L., Artzt, K. et al. Inversion in the H–2 complex of t-haplotypes in mice. Nature 306, 380–383 (1983). https://doi.org/10.1038/306380a0
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DOI: https://doi.org/10.1038/306380a0
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