Abstract
Mouse monoclonal anti-dansyl antibodies with the same antigen-binding sites but different heavy chain constant regions were generated. The extent of segmental flexibility in times of nanoseconds and the capacity to fix complement were greatest for IgG2b, intermediate for IgG2a, and least for IgG1 and IgE. Hence, the effector functions of immunoglobulin isotypes may be controlled in part by the freedom of movement of their Fab arms.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Noelken, M. E., Nelson, C. A., Buckley, C. E. & Tanford, C. J. biol. Chem. 240, 218–224 (1965).
Valentine, R. C. & Green, N. M. J. molec. Biol. 27, 615–617 (1967).
Feinstein, A. & Rowe, A. J. Nature 205, 147–149 (1965).
Marquart, M., Deisenhofer, J., Huber, R. & Palm, W. J. molec. Biol. 141, 369–391 (1980).
Amzel, L. M. & Poljak, R. J. A. Rev. Biochem. 48, 961–967 (1979).
Silverton, E. W., Navia, M. A. & Davies, D. M. Proc. natn. Acad. Sci. U.S.A. 74, 5140–5144 (1977).
Yguerabide, J., Epstein, H. E. & Stryer, L. J. molec. Biol. 51, 573–590 (1970).
Cathou, R. E. Compreh. Immun. 5, 37–83 (1978).
Hanson, D. C., Yguerabide, J. & Schumaker, V. N. Biochemistry 20, 6842–6852 (1981).
Cebra, J. J. et al. Proc. Robert A. Welch Foundation XVIII Immunochem, Houston, Texas (ed. Milligan, W. O.) 67–96 (Welch Foundation, (1975).
Dudich, E., Nezlin, R. S. & Franek, F. FEBS Lett. 89, 89–92 (1978).
Coffino, P., Laskov, R. & Scharff, M. D. Science 167, 186–188 (1970).
Liesegang, B., Radbruch, A. & Rajewsky, K. Proc. natn. Acad. Sci. U.S.A. 75, 3901–3905 (1978).
Dangl, J. L., Parks, D. R., Oi, V. T. & Herzenberg, L. A. Cytometry 2, 395–401 (1982).
Stryer, L. Science 162, 526–533 (1968).
Reidler, J. et al. J. molec. Biol. 158, 739–746 (1982).
O'Farrell, P. Z., Goodman, H. M. & O'Farrell, P. H. Cell 12, 1133–1142 (1977).
Oi, V. T., Bryan, V. M., Herzenberg, L. A. & Herzenberg, L. A. J. exp. Med. 151, 1260–1274 (1980).
Hickman, S. & Kornfeld, S. J. Immun. 121, 990–996 (1978).
Edelman, G. M. et al. Proc. natn. Acad. Sci. U.S.A. 63, 78–85 (1969).
Kornfeld, R. & Kornfeld, S. A. Rev. Biochem. 45, 217–237 (1976).
Yguerabide, J. Meth. Enzym. 26C, 498–577 (1972).
Ishida, N., Ueda, S., Hayashida, H., Mitata, T. & Honjo, T. EMBO J. 1, 1117–1123 (1982).
Chan, L. M. & Cathou, R. E. J. molec. Biol. 112, 653–656 (1977).
Bengali, Z. H., Das, S. R. & Levine, P. H. J. immun. Meth. 33, 63–00 (1980).
Oi, V. T., Morrison, S. L., Herzenberg, L. A. & Berg, P. Proc. natn. Acad. Sci. U.S.A. 80, 825–829 (1983).
Rice, D. & Baltimore, D. Proc. natn. Acad. Sci. U.S.A. 79, 7862–7865 (1982).
Miller, R. A. & Levy, R. Lancet i, 226–229 (1981).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Oi, V., Vuong, T., Hardy, R. et al. Correlation between segmental flexibility and effector function of antibodies. Nature 307, 136–140 (1984). https://doi.org/10.1038/307136a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/307136a0
This article is cited by
-
Structural insights into the bi-specific cross-over dual variable antibody architecture by cryo-EM
Scientific Reports (2023)
-
Biophysical characterization of dynamic structures of immunoglobulin G
Biophysical Reviews (2020)
-
Human immunoglobulin G hinge regulates agonistic anti-CD40 immunostimulatory and antitumour activities through biophysical flexibility
Nature Communications (2019)
-
Developing the IVIG biomimetic, Hexa-Fc, for drug and vaccine applications
Scientific Reports (2015)
-
Human immunoglobulin E flexes between acutely bent and extended conformations
Nature Structural & Molecular Biology (2014)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.