Abstract
The site of integration of transgenes in the host genome can affect levels of expression1 and occasionally confer ectopic patterns of expression on otherwise tissue-specific genes2. We describe here a line of mice in which an hsp68-lacZ transgene is expressed in unstressed developing neural tissue and where the transgene insertion has caused a mutation of a neural tissue-specific gene, dystonia musculorum (dt). This coincidence suggests that expression of the hsp68-lacZ construct may be controlled directly by cis-acting regulatory sequences that normally control the developmental expression of the dt gene. Such constructs may serve as useful tools for identifying new tissue-specific enhancers and their associated genes3,4.
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References
Palmiter, R. D. & Brinster, R. L. A. Rev. Genet. 20, 465–499 (1986).
Lacy, E., Roberts, S., Evans, E. P., Burtenshaw, M. D. & Costantini, F. Cell 34, 343–358 (1983).
O'Kane, C. J. & Gehring, W. J. Proc. natn. Acad. Sci. U.S.A. 84, 9123–9127 (1987).
Allan, N. D. et al. Nature 33, 852–855 (1988).
Duchen, L. W. Adv. Neurol. 14, 353–365 (1976).
Duchen, L. W. & Strich, S. J. Brain 87, 367–378 (1984).
Riker, D. K., Messer, A. & Roth, R. H. J. Neurochem. 37, 649–654 (1981).
Zeman, W. Adv. Neurol. 14, 91–102 (1976).
Dannenberg, A. M. & Suga, M. In Methods for Studying Mononudear Phagocytes (eds Adams, D., Edelson, P. & Koren, M.) 375–396 (Academic, New York, 1981).
Estable-Puig, J. F., Bauer, W. C. & Blumberg, J. M. J. Neuropath, exp. Neurol. 24, 531–535 (1965).
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Kothary, R., Clapoff, S., Brown, A. et al. A transgene containing lacZ inserted into the dystonia locus is expressed in neural tube. Nature 335, 435–437 (1988). https://doi.org/10.1038/335435a0
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DOI: https://doi.org/10.1038/335435a0
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