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Pathway of phosphatidylinositol(3,4,5)-trisphosphate synthesis in activated neutrophils

Nature volume 351, pages 33–39 (1991)Cite this article

Abstract

Neutrophils activated by the formyl peptide f-Met-LeuPhe transiently accumulate a small subset of highly polar inositol lipids. A similar family of lipids also appear in many other cells in response to a range of growth factors and activated oncogenes, and are presumed to be the direct or indirect products of 3-phosphatidylinositol kinase. The structures of these lipids are shown to be phos-phatidylinositol 3-phosphate, phosphatidylinositol-(3,4)bisphosphate and phosphatidylinositol-(3,4,5)trisphosphate, and we present evidence that in intact neutrophils a phosphatidylinositol-(4,5)bisphosphate-3-kinase seems to be the focal point through which agonists stimulate the formation of 3-phosphorylated inositol lipids.

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Author information

Author notes

  1. K. T. Hughes: Amersham International, Whitchurch, Cardiff CF4 7YT, UK

Authors and Affiliations

  1. Biochemistry Department, AFRC Institute of Animal Physiology & Genetics Research, Cambridge Research Station, Babraham Hall, Cambridge, CB2 4AT, UK

    L. R. Stephens, K. T. Hughes & R. F. Irvine

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  1. L. R. Stephens
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  2. K. T. Hughes
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  3. R. F. Irvine
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Stephens, L., Hughes, K. & Irvine, R. Pathway of phosphatidylinositol(3,4,5)-trisphosphate synthesis in activated neutrophils. Nature 351, 33–39 (1991). https://doi.org/10.1038/351033a0

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