Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Activation of Raf-1 by 14-3-3 proteins

Nature volume 371, pages 612–614 (1994)Cite this article

Abstract

THE protein Raf-1, a key mediator of mitogenesis and differentia-tion, associates with p21*"a5 (refs 1-3). However, the regulation of the serine/threonine kinase activity of Raf-1 is still not understood4 13. Using the yeast two-hybrid system8'14"16, we identi-fied two structurally related proteins that interact with the amino-terminal region of Raf-1. These proteins, 14-3-3 £ (PLA2) and 14-3-3 p (HS1), are members of the 14-3-3 family of proteins17'23. Expression of 14-3-3 proteins in Xenopm oocytes enhanced Raf-1 activity and promoted Raf-1-dependent oocyte maturation. A dominant negative mutant of Raf-1 blocked the effects of 14-3-3 protein.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Rapp, U. R. et al. Oncogene 6, 495–500 (1991).

    CAS  Google Scholar 

  2. Kolch, W., Heidecker, G., Lloyd, P. & Rapp, U. R. Nature 349, 426–428 (1991).

    Article  ADS  CAS  Google Scholar 

  3. MacNicol, A. M., Muslin, A. J. & Williams, L. T. Cell 73, 571–583 (1993).

    Article  CAS  Google Scholar 

  4. Dent, P. et al. Science 257, 1404–1407 (1992).

    Article  ADS  CAS  Google Scholar 

  5. Howe, L. R. et al. Cell 71, 335–342 (1992).

    Article  CAS  Google Scholar 

  6. Kyriakis, J. M. et al. Nature 358, 417–421 (1992).

    Article  ADS  CAS  Google Scholar 

  7. Moodie, S. A., Willumsen, B. M., Weber, M. J. & Wolfman, A. Science 260, 1658–1661 (1993).

    Article  ADS  CAS  Google Scholar 

  8. VanAelst, L., Barr, M., Marcus, S., Polverino, A. & Wigler, M. Proc. natn. Acad. Sci. U.S.A. 90, 6213–6217 (1993).

    Article  ADS  CAS  Google Scholar 

  9. Vojtek, A. B., Hollenberg, S. M. & Cooper, J. A. Cell 74, 205–214 (1993).

    Article  CAS  Google Scholar 

  10. Warne, P. H., Rodriguez, V. & Downward, J. Nature 364, 352–355 (1993).

    Article  ADS  CAS  Google Scholar 

  11. Zhang, X. et al. Nature 364, 308–313 (1993).

    Article  ADS  CAS  Google Scholar 

  12. Leevers, S. J., Paterson, C. J. & Marshall, C. J. Nature 369, 411–414 (1994).

    Article  ADS  CAS  Google Scholar 

  13. Stokoe, D., Macdonald, S. G., Cadwallader, K., Symons, M. & Hancock, J. F. Science 264, 1463–1467 (1994).

    Article  ADS  CAS  Google Scholar 

  14. Fields, S. & Song, O. Nature 340, 245–247 (1989).

    Article  ADS  CAS  Google Scholar 

  15. Harper, J. W. et al. Cell 75, 805–816 (1993).

    Article  CAS  Google Scholar 

  16. Hannon, G. J., Demitrick, D. & Beach, D. Genes Dev. 7, 2378–2391 (1993).

    Article  CAS  Google Scholar 

  17. Aitken, A. et al. Trends biochem. Sci. 17, 498–501 (1992).

    Article  CAS  Google Scholar 

  18. Nielsen, P. J. Biochim. biophys. Acta 1088, 425–428 (1991).

    Article  CAS  Google Scholar 

  19. Leffers, H. et al. J. molec. Biol. 231, 982–998 (1993).

    Article  CAS  Google Scholar 

  20. Zupan, L. A., Steffens, D. L., Berry, C. A., Landt, M. & Gross, R. W. J. biol. Chem. 267, 8707–8710 (1992).

    CAS  PubMed  Google Scholar 

  21. Ford, J. C. et al. Science 262, 533–535 (1994).

    Article  ADS  Google Scholar 

  22. Du, X., Harris, S. J., Tetaz, T. J., Ginsberg, M. H. & Berndt, M. C. J. biol. Chem. 269, 18287–18290 (1994).

    CAS  PubMed  Google Scholar 

  23. Pallas, D. C. et al. Science 262, 535–537 (1994).

    Article  ADS  Google Scholar 

  24. Kikuchi, A. & Williams, J. J. biol. Chem. 269, 20054–20059 (1994).

    CAS  PubMed  Google Scholar 

  25. Fabian, J. R., Morrison, D. K. & Daar, I. O. J. Cell Biol. 112, 645–652 (1993).

    Article  Google Scholar 

  26. Muslin, A. J., MacNicol, A. M. & Williams, L. T. Molec. cell. Biol. 13, 4197–4202 (1993).

    Article  CAS  Google Scholar 

  27. Smith, L. D. Development 107, 685–699 (1989).

    CAS  PubMed  Google Scholar 

  28. Meijer, L. et al. EMBO J. 8, 2275–3382 (1989).

    Article  CAS  Google Scholar 

  29. Breeden, L. & Nasmyth, K. Cold Spring Harb. Symp. quant. Biol. 50, 643–650 (1985).

    Article  CAS  Google Scholar 

  30. Durfee, L. et al. Genes Dev. 7, 555–569 (1993).

    Article  CAS  Google Scholar 

Download references

Author information

Author notes

  1. Anthony J. Muslin

    Present address: Cardiology Division, Department of Medicine, Jewish Hospital, Washington University Medical Center, St Louis, MO, 63110, USA

  2. Angus M. MacNicol

    Present address: University of Chicago, 5841 South Maryland Avenue, Chicago, IL, 60637, USA

  3. Wendy J. Fantl & Lewis T. Williams

    Present address: Chiron Corporation, Emeryville, CA, 94608, USA

  4. Richard W. Grosst: Division of Bioorganic Chemistry and Molecular Pharmacology, Washington University School of Medicine, St Louis, Missouri 63110, USA

Authors and Affiliations

  1. Daiichi Research Center, Cardiovascular Research Institute, Department of Medicine, University of California, San Francisco, California, 94143, USA

    Wendy J. Fantl, Anthony J. Muslin, Akira Kikuchi, Jay A. Martin, Angus M. MacNicol, Richard W. Grosst & Lewis T. Williams

Authors
  1. Wendy J. Fantl
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Anthony J. Muslin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Akira Kikuchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jay A. Martin
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Angus M. MacNicol
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Richard W. Grosst
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Lewis T. Williams
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fantl, W., Muslin, A., Kikuchi, A. et al. Activation of Raf-1 by 14-3-3 proteins. Nature 371, 612–614 (1994). https://doi.org/10.1038/371612a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/371612a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing