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A presynaptic inositol-5-phosphatase

Nature volume 379, pages 353–357 (1996)Cite this article

Abstract

SYNAPTOJANIN is a nerve terminal protein of relative molecular mass 145,000 which appears to participate with dynamin in synaptic vesicle recycling1,2. The central region of synaptojanin defines it as a member of the inositol-5-phosphatase family, which includes the product of the gene that is defective in the oculocerebrorenal syndrome of Lowe3–7. Synaptojanin has 5-phosphatase activity and its amino-terminal domain is homologous with the yeast protein Sacl (Rsdl), which is genetically implicated in phospholipid metabolism and in the function of the actin cytoskeleton8–10. The carboxy terminus, which is of different lengths in adult and developing neurons owing to the alternative use of two termination sites, is proline-rich, consistent with the reported interaction of synaptojanin with the SH3 domains of Grb2 (refs 1, 2). Synaptojanin is the only other major brain protein besides dynamin that binds the SH3 domain of amphi-physin, a presynaptic protein with a putative function in endo-cytosis11–14. Our results suggest a link between phosphoinositide metabolism and synaptic vesicle recycling.

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Author notes

  1. Peter S. McPherson

    Present address: Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, H3A 2B4, Canada

  2. Pietro De Camilli: To whom correspondence should be addressed.

Authors and Affiliations

  1. Department of Cell Biology and Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, Connecticut, 06510, USA

    Peter S. McPherson, Elizabeth P. Garcia, Vladimir I. Slepnev, Carol David, Xiaomei Zhang, Detlev Grabs, Rudolf Bauerfeind, Yasuo Nemoto & Pietro De Camilli

  2. Department of Neurology and Neurosurgery, Montreal Neurological lnstitute, McGill University, Montreal, H3A 2B4, Canada

    Wayne S. Sossini

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  4. Carol David
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McPherson, P., Garcia, E., Slepnev, V. et al. A presynaptic inositol-5-phosphatase. Nature 379, 353–357 (1996). https://doi.org/10.1038/379353a0

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