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Transgenic expression of green fluorescence protein can cause dilated cardiomyopathy

Nature Medicine volume 6, pages 482–483 (2000)Cite this article

To the editor

A common approach for determining the molecular mechanisms of cardiac hypertrophy and failure has been the use of transgenic mice1. Many transgenic models of dilated cardiac hypertrophy have been reported2. In these models, the non-transgenic littermates are generally used as controls. However, because non-specific effects of a transgene can be dose- and time-dependent, non-transgenic littermates might not be a sufficient control for transgenic experiments.

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Figure 1: Confirmation of GFP expression by western blot analysis, and cardiac pathology.

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Authors and Affiliations

  1. Cardiovascular division, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, 02215, Massachusetts , USA

    Weei-Yuarn Huang, Pamela S. Douglas & Seigo Izumo

  2. The Center for Blood Research, 200 Longwood Avenue, Boston, 02215, Massachusetts, USA

    Jose Aramburu

Authors
  1. Weei-Yuarn Huang
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  2. Jose Aramburu
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  3. Pamela S. Douglas
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  4. Seigo Izumo
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Correspondence to Seigo Izumo.

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Huang, WY., Aramburu, J., Douglas, P. et al. Transgenic expression of green fluorescence protein can cause dilated cardiomyopathy. Nat Med 6, 482–483 (2000). https://doi.org/10.1038/74914

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