Abstract
Glycosylation modifies protein activities in various biological processes. Here, we report the functions of a novel UDP–sugar transporter (UST74C, an alternative name for Fringe connection (Frc)) localized to the Golgi apparatus in cellular signalling of Drosophila. Mutants in the frc gene exhibit phenotypes resembling wingless and Notch mutants. Both Fringe-dependent and Fringe-independent Notch pathways are affected, and both glycosylation and proteolytic maturation of Notch are defective in mutant larvae. The results suggest that changes in nucleotide–sugar levels can differently affect Wingless and two distinct aspects of Notch signalling.
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Acknowledgements
We thank S. Artavanis-Tsakonas, M. Freeman, Y. Hiromi, K. Irvine, Y.-N. Jan, A. Martinez-Arias, K. Matsuno, the Bloomington Stock Center, the Developmental Studies Hybridoma Bank and Umea Stock Center for antibodies and flies. We also thank Y. Hiromi, M. Iwanami, R. Kannagi, T. Saito, H. Segawa, H. Takeuchi, N. Taniguchi, H. Nakato and the members of the Invertebrate laboratory at NIG for their technical advice and critical comments. This work was supported by grants to S.G. and S.H. from the Ministry of Education, Science, Sports and Culture, and to S.H. from the Japan Society for the Promotion of Sciences (Research for the Future).
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Goto, S., Taniguchi, M., Muraoka, M. et al. UDP–sugar transporter implicated in glycosylation and processing of Notch. Nat Cell Biol 3, 816–822 (2001). https://doi.org/10.1038/ncb0901-816
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DOI: https://doi.org/10.1038/ncb0901-816
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