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High resolution visual mapping of stretched DNA by fluorescent hybridization

Nature Genetics volume 5, pages 17–21 (1993)Cite this article

Abstract

We describe a method for stretching DNA, which, when combined with fluorescent hybridization procedures, forms a new mapping technology that produces a high resolution, vivid, multi–colour image and map. Restriction fragments and cosmid probes were successfully mapped by this procedure with validation by standard restriction mapping. A long range map of a >200 kilobase region containing five copies of the amplified dihydrofolate reductase gene was easily generated within two days. This DNA mapping procedure offers a significant and rapid alternative to a variety of standard mapping procedures.

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  1. The Cancer Therapy and Research Center, 8122 Datapoint Drive, San Antonio, Texas, 78229, USA

    Irma Parra & Bradford Windle

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  1. Irma Parra
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  2. Bradford Windle
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Parra, I., Windle, B. High resolution visual mapping of stretched DNA by fluorescent hybridization. Nat Genet 5, 17–21 (1993). https://doi.org/10.1038/ng0993-17

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