Abstract
Ideal gene therapy vectors would be delivered intravenously to transfect only specific cells. Existing vectors only transfect cells in vivo in a manner determined by blood flow and the site of introduction. As a general and systematic approach for generating Cell–targeting ligands for gene therapy vectors, we have used peptide–presenting phage libraries to select peptides that bind and enter several different Cell types. Because of their small size, cell–binding peptides such as these could be incorporated into biological or physical gene therapy vectors. In addition, peptide–presenting phage themselves may also be candidates for gene therapy vectors.
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Barry, M., Dower, W. & Johnston, S. Toward cell–targeting gene therapy vectors: Selection of cell–binding peptides from random peptide–presenting phage libraries. Nat Med 2, 299–305 (1996). https://doi.org/10.1038/nm0396-299
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DOI: https://doi.org/10.1038/nm0396-299
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