Abstract
We have generated a recombinant Mos1 transposon that can insert up to 45-kb transgenes into the Caenorhabditis elegans genome. The minimal Mos1 transposon (miniMos) is 550 bp long and inserts DNA into the genome at high frequency (∼60% of injected animals). Genetic and antibiotic markers can be used for selection, and the transposon is active in C. elegans isolates and Caenorhabditis briggsae. We used the miniMos transposon to generate six universal Mos1-mediated single-copy insertion (mosSCI) landing sites that allow targeted transgene insertion with a single targeting vector into permissive expression sites on all autosomes. We also generated two collections of strains: a set of bright fluorescent insertions that are useful as dominant, genetic balancers and a set of lacO insertions to track genome position.
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Acknowledgements
We thank B. Waterston (University of Washington), A. Sapir and P. Sternberg (California Institute of Technology), and the NemaGENETAG consortium for strains; B. Meyer (UC Berkeley) and P. Meister (University of Bern) for validating lacO insertions; the J. Chin (MRC, University of Cambridge), D. Dupuy (University of Bordeaux), B. Lehner (EMBL-CRG, Systems Biology Unit, Barcelona) and G. Seydoux (John Hopkins University) labs for plasmids; M. Maduro (UC Riverside) for improving mosSCI insertion frequency; and K. Hoe for expert technical assistance. Some strains were provided by the Caenorhabditis Genetics Center (CGC), which is funded by US National Institutes of Health (NIH) Office of Research Infrastructure Programs (P40 OD010440). This work was supported by the Carlsberg Foundation (C.F.-J.), NIH grant 1R01GM095817 (E.M.J.), US National Science Foundation grant NSF IOS-0920069 (E.M.J.) and the Howard Hughes Medical Institute (E.M.J.). The Mosmid engineering work was supported by the Max Planck Society (MPG) Initiative “BAC TransgeneOmics” and the NIH ModENCODE project. Work in the laboratory of D.G.M. was supported by the Canadian Institute for Health Research. Work in the laboratory of D.G.M. was supported by the Canadian Institute for Health Research and the Canadian Institute for Advanced Research.
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C.F.-J. designed experiments under the supervision of E.M.J. and M.W.D. C.F.-J., M.S., A.P., J.T., M.L. and S.F. performed the research. C.F.-J. performed molecular biology, injections, imaging and genetics; M.L. generated mapping strains; M.S. and A.P. performed fosmid recombineering; and J.T., S.F. and D.G.M. performed comparative genome hybridization. C.F.-J. and E.M.J. wrote the paper with input from all coauthors.
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Supplementary Figures 1–9, Supplementary Note and Supplementary Protocols (PDF 1861 kb)
Supplementary Table 1
Strains, Plasmids, Oligos and Universal insertion sites (XLSX 318 kb)
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Frøkjær-Jensen, C., Davis, M., Sarov, M. et al. Random and targeted transgene insertion in Caenorhabditis elegans using a modified Mos1 transposon. Nat Methods 11, 529–534 (2014). https://doi.org/10.1038/nmeth.2889
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DOI: https://doi.org/10.1038/nmeth.2889
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