Key Points
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Male infertility accounts for 5–7% of infertility in human couples. Treatment so far is limited and, in most cases, the couples have to resort to IVF techniques that circumvent rather than treat this problem.
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Many environmental, behavioural and genetic factors affect male fertility, with the genetic contribution estimated at 60%.
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Spermatogenesis is a complex process that involves stem-cell renewal, genome reorganization and genome repackaging that culminates in the production of motile gametes.
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Because of the complex interaction between the soma and the germline during spermatogenesis, the process cannot be recapitulated in vitro.
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Although there are differences between spermatogenesis in mouse and man, the similarities are sufficient to use the mouse as a model of human spermatogenesis.
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Mouse mutants have been invaluable in obtaining information on all aspects of spermatogenesis, at both fetal and post-natal stages.
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Although we have a long way to go before we can treat male infertility, techniques developed in the mouse, such as germ-cell transplantation have already found their way to the clinic.
Abstract
Spermatogenesis is a complex process that involves stem-cell renewal, genome reorganization and genome repackaging, and that culminates in the production of motile gametes. Problems at all stages of spermatogenesis contribute to human infertility, but few of them can be modelled in vitro or in cell culture. Targeted mutagenesis in the mouse provides a powerful method to analyse these steps and has provided new insights into the origins of male infertility.
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Glossary
- RNAi
-
A phenomenon in which the expression of a gene is temporarily inhibited when a double-stranded complementary RNA is introduced into the organism.
- SPERMATOGONIUM
-
A diploid male germ cell that divides mitotically to produce stem cells or cells that enter meiosis.
- PITUITARY GLAND
-
An endocrine gland that is located at the base of the brain, and that produces hormones, such as growth hormone, luteinizing hormone, follicle stimulating hormone and thyroid stimulating hormone.
- SEMINIFEROUS TUBULE
-
A structural unit in the adult testis; it consists of somatic Sertoli cells and germ cells at several developmental stages.
- SPERMIOGENESIS
-
The stage of spermatogenesis during which spermatids undergo cell remodelling and DNA compaction.
- SPERMIATION
-
The release of mature spermatozoa from the surface of the Sertoli cell into the lumen of the seminiferous tubule.
- SPERMATOCYTE
-
A cell that is progressing through meiotic prophase.
- SPERMATID
-
A post-meiotic, haploid germ cell.
- ADRENAL GLAND
-
A gland located on top of the kidney that produces hormones that regulate aspects of physiology, such as the heart rate and blood pressure.
- SYNAPTONEMAL COMPLEX
-
A structure that holds paired chromosomes together during prophase 1 of meiosis and that promotes genetic recombination.
- CHECKPOINT
-
Quality-control points during the cell cycle that ensure that each phase of the cycle is completed successfully before the process is allowed to continue.
- PROTAMINE
-
Small arginine-rich proteins that are deposited on DNA towards the end of sperminogenesis.
- ACROSOME
-
This structure arises from the Golgi complex. It first develops in spermatids and eventually forms a cap structure over the nucleus in the mature spermatozoa.
- TRANSITION PROTEINS
-
Small basic nuclear proteins that replace histones on germ cell DNA. When DNA compaction begins they, in turn, are replaced by protamines.
- HAPLOINSUFFICIENCY
-
A phenotype that arises in diploid organisms owing to the loss of one functional copy of a gene.
- GONOCYTES
-
Once the gonad has differentiated into a testis by forming seminiferous cords, primitive germ cells are referred to as gonocytes.
- GENE TRAP
-
A DNA construct that contains a reporter gene sequence downstream of a splice acceptor site that is capable of integrating into random chromosomal locations in mouse. Integration of the gene trap into an intron allows the expression of a new mRNA containing one or more upstream exons followed by the reporter gene.
- KNOCKING DOWN
-
Removing function of a gene in a transient way, such as by RNAi.
- AUTOLOGOUS GERM-CELL TRANSPLANTATION
-
Transplantation of spermatogonial stem cells (As) from one animal into that of a recipient in which germ cells are missing.
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Cooke, H., Saunders, P. Mouse models of male infertility. Nat Rev Genet 3, 790–801 (2002). https://doi.org/10.1038/nrg911
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DOI: https://doi.org/10.1038/nrg911
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