Abstract
Removal of atypical PKC blocks NGF-induced differentiation of PC12 cells.* We now examine the consequences that overexpression of atypical PKCs had upon NGF responses. PC12 cells were stably transfected with either PKC-ι or PKC-ζ. Overexpression of atypical PKCs markedly enhanced NGF- induced neurite outgrowth as well as enhanced NGF-stimulated JNK kinase. Cotransfection of HA-JNK1 along with increasing concentrations of PKC-ι, resulted in dose-dependent phosphorylation of GST c-Jun (1–79). NGF treatment of PC12 cells resulted in activation of NF-κB. In comparison, overexpression of atypical PKC-ι was by itself sufficient to activate NF-κB and shift the kinetics of NGF-induced κB activity. Furthermore, transfection of full-length antisense PKC-ι blocked basal and NGF-stimulated NF-κB. Differentiated and undifferentiated PC12 cells overexpressing atypical PKC-ι were protected from serum deprivation-induced cell death. Collectively, these findings demonstrate that atypical PKC-ι lies in a pathway that regulates NF-κB and contributes to both neurotrophin-mediated differentiation and survival signaling.
Similar content being viewed by others
Article PDF
Author information
Authors and Affiliations
Corresponding author
Additional information
Edited by C. Thiele
Rights and permissions
About this article
Cite this article
Wooten, M., Seibenhener, M., Zhou, G. et al. Overexpression of atypical PKC in PC12 cells enhances NGF-responsiveness and survival through an NF-κB dependent pathway. Cell Death Differ 6, 753–764 (1999). https://doi.org/10.1038/sj.cdd.4400548
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/sj.cdd.4400548
Keywords
This article is cited by
-
Macrophages-aPKCɩ-CCL5 Feedback Loop Modulates the Progression and Chemoresistance in Cholangiocarcinoma
Journal of Experimental & Clinical Cancer Research (2022)
-
Identification of genes and gene pathways associated with major depressive disorder by integrative brain analysis of rat and human prefrontal cortex transcriptomes
Translational Psychiatry (2015)
-
Nerve Growth Factor-Mediated Regulation of Low Density Lipoprotein Receptor-Related Protein Promoter Activation
Cellular and Molecular Neurobiology (2013)