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With a little help from a friend: increasing HIV transduction of monocyte-derived dendritic cells with virion-like particles of SIVMAC

Gene Therapy volume 13, pages 991–994 (2006)Cite this article

Abstract

Modification of dendritic cells (DCs) is a promising avenue for gene therapy purposes, given the versatility and the multiplicity of functions of these cells. In this study, we show that preincubation of monocyte-derived DCs with low amounts of non-infectious virion-like particles derived from the simian immunodeficiency virus (SIVMAC VLPs) increases up to 10-fold the efficiency of transduction by HIV-1 lentiviral vectors at low multiplicity of infections yielding up to 90% of transduced cells, in the absence of alterations of DCs behavior. This effect is restricted to DCs and specified by the viral accessory protein Vpx. Thus, preincubation with empty VLPs of SIVMAC can be used in transduction protocols to increase the efficacy of HIV-1-mediated modification of DCs.

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Acknowledgements

We are indebted to Dr Pavlakis for the SIV Gag construct. AC is financially supported by ANRS, Sidaction and NIH, and JLD from ANRS, and the TRIoH program of the EC.

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Authors and Affiliations

  1. INSERM U412, Ecole Normale Supérieure de Lyon, IFR 128 BioSciences Lyon-Gerland, Lyon, France

    C Goujon, L Jarrosson-Wuillème, J-L Darlix & A Cimarelli

  2. Etablissement Français du Sang, Lyon, France

    J Bernaud & D Rigal

Authors
  1. C Goujon
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  2. L Jarrosson-Wuillème
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  3. J Bernaud
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  4. D Rigal
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  5. J-L Darlix
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  6. A Cimarelli
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Correspondence to A Cimarelli.

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Goujon, C., Jarrosson-Wuillème, L., Bernaud, J. et al. With a little help from a friend: increasing HIV transduction of monocyte-derived dendritic cells with virion-like particles of SIVMAC. Gene Ther 13, 991–994 (2006). https://doi.org/10.1038/sj.gt.3302753

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  • DOI: https://doi.org/10.1038/sj.gt.3302753

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