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The cloning, mapping and expression of a novel gene, BRL, related to the AF10 leukaemia gene

Oncogene volume 18, pages 7442–7452 (1999)Cite this article

Abstract

The MLL gene is reciprocally translocated with one of a number of different partner genes in a proportion of human acute leukaemias. The precise mechanism of oncogenic transformation is unclear since most of the partner genes encode unrelated proteins. However, two partner genes, AF10 and AF17 are related through the presence of a cysteine rich region and a leucine zipper. The identification of other proteins with these structures will aid our understanding of their role in normal and leukaemic cells. We report the cloning of a novel human gene (BRL) which encodes a protein containing a cysteine rich region related to that of AF10 and AF17 and is overall most closely related to the previously known protein BR140. BRL maps to chromosome 22q13 and shows high levels of expression in testis and several cell lines. The deduced protein sequence also contains a bromodomain, four potential LXXLL motifs and four predicted nuclear localization signals. A monoclonal antibody raised to a BRL peptide sequence confirmed its widespread expression as a 120 Kd protein and demonstrated localization to the nucleus within spermatocytes.

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Acknowledgements

This work was funded by the Camilla Samuel Research Fellowship held by P McCullagh at the ICRF. We gratefully acknowledge the support and advice of Professor TA Lister. We are grateful for the support of Jane Steele and Maggie Stubbs of the Hybridoma Laboratory, ICRF. The Jurkat cell line cDNA library was a gift from Dr J Dunne (ICRF). We thank Miss V Lewis (medical student) for technical assistance.

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Authors and Affiliations

  1. Department of Medical Oncology, Imperial Cancer Research Fund, St Bartholomew's and the Royal London School of Medicine and Dentistry, Charterhouse Square, London, 6BQ, ECIM, UK

    Paul McCullagh, Tracy Chaplin, Joanne Meerabux, Demetrios Grenzelias, Debra Lillington, Armando Gregorini, Vaskar Saha & Bryan D Young

  2. In Situ Hybridization Service, Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, London, WC2A 3PX, UK

    Richard Poulsom

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McCullagh, P., Chaplin, T., Meerabux, J. et al. The cloning, mapping and expression of a novel gene, BRL, related to the AF10 leukaemia gene. Oncogene 18, 7442–7452 (1999). https://doi.org/10.1038/sj.onc.1203117

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