Issue 39, 2013
From the journal:

Chemical Communications

Artificial repressors for controlling gene expression in bacteria†‡

Mark C. Politz,§ab   Matthew F. Copeland§ab  and  Brian F. Pfleger*ab  

* Corresponding authors

a University of Wisconsin-Madison, Department of Chemical and Biological Engineering, 3629 Engineering Hall, 1415 Engineering Dr Madison, WI, USA
E-mail: pfleger@wisc.edu
Fax: +01 608 262 5434
Tel: +01 608 890 1940

b U. S. Department of Energy Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, 1550 Linden Drive Madison, WI, USA

Abstract

Transcriptional repression is a common approach to control gene expression in synthetic biology applications. Here, an engineered DNA binding protein based upon a transcription activator-like effector (TALE) scaffold was shown to outperform LacI in blocking transcription from a promoter and to repress expression of a downstream gene in an operon.

Graphical abstract: Artificial repressors for controlling gene expression in bacteria

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Article information

DOI
https://doi.org/10.1039/C2CC37107C
Article type
Communication
Submitted
29 Sep 2012
Accepted
16 Nov 2012
First published
19 Nov 2012

Chem. Commun., 2013,49, 4325-4327

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Artificial repressors for controlling gene expression in bacteria

M. C. Politz, M. F. Copeland and B. F. Pfleger, Chem. Commun., 2013, 49, 4325 DOI: 10.1039/C2CC37107C

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