Issue 80, 2014
From the journal:

RSC Advances

Paper-based molecular diagnostic for Chlamydia trachomatis†

Jacqueline C. Linnes,a   Andy Fan,a   Natalia M. Rodriguez,a   Bertrand Lemieux,b   Huimin Kongb  and  Catherine M. Klapperich*a  

* Corresponding authors

a Biomedical Engineering, Boston, University, Boston, MA, USA
E-mail: catherin@bu.edu
Fax: +1 617-353-6766
Tel: +1 617-358-0253

b BioHelix Corporation, Beverly, MA, USA

Abstract

Herein we show the development of a minimally instrumented paper-based molecular diagnostic for point of care detection of sexually transmitted infections caused by Chlamydia trachomatis. This new diagnostic platform incorporates cell lysis, isothermal nucleic acid amplification, and lateral flow visual detection using only pressure and heat sources, eliminating the need for expensive laboratory equipment. This paper-based test can be performed in less than one hour and has a clinically relevant limit of detection that is 100× more sensitive than current rapid immunoassays used for chlamydia diagnosis.

Graphical abstract: Paper-based molecular diagnostic for Chlamydia trachomatis

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Article information

DOI
https://doi.org/10.1039/C4RA07911F
Article type
Paper
Submitted
31 Jul 2014
Accepted
29 Aug 2014
First published
29 Aug 2014

RSC Adv., 2014,4, 42245-42251

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Paper-based molecular diagnostic for Chlamydia trachomatis

J. C. Linnes, A. Fan, N. M. Rodriguez, B. Lemieux, H. Kong and C. M. Klapperich, RSC Adv., 2014, 4, 42245 DOI: 10.1039/C4RA07911F

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