Issue 37, 2019
From the journal:

Nanoscale

Nanodiamond uptake in colon cancer cells: the influence of direction and trypsin-EDTA treatment†

Alina Sigaeva, ORCID logo *a   Aryan Morita, ORCID logo ab   Simon R. Hemelaara  and  R. Schirhagl ORCID logo *a  

* Corresponding authors

a University Medical Center Groningen, Antonius Deusinglaan 1, 9713 AV Gronigen, The Netherlands
E-mail: romana.schirhagl@gmail.com, aosigaeva@gmail.com

b Department of Dental Biomedical Sciences, Faculty of Dentistry, Universitas Gadjah, Mada, Jl Denta 1, 55281 Yogyakarta, Indonesia

Abstract

Nanoparticles are routinely used in cell biology. They deliver drugs or function as labels or sensors. For many of these applications it is essential that the nanoparticles enter the cells. While some cell types readily ingest all kinds of particles, others just don't. We report that uptake can be enhanced for some cells if the particles are administered from the basolateral side of the cells (in this case from below). Compared to apical uptake (from above), we report an 8-fold increase in the number of fluorescent nanodiamonds internalized by the colon cancer cell line HT29. Up to 96% of the cells treated by a modified protocol contain at least one nanodiamond, whereas in the control group we could observe nanodiamonds in less than half of the cells. We were also able to show that simple treatment of cell clusters with trypsin-EDTA leads to the same enhancement of the nanodiamond uptake as seeding the cells on top of the nanoparticles. Although our study is focused on nanodiamonds in HT29 cells, we believe that this method could also be applicable for other nanoparticles and cells with a specific directionality.

Graphical abstract: Nanodiamond uptake in colon cancer cells: the influence of direction and trypsin-EDTA treatment

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Article information

DOI
https://doi.org/10.1039/C9NR04228H
Article type
Paper
Submitted
17 May 2019
Accepted
24 Aug 2019
First published
13 Sep 2019
This article is Open Access
Creative Commons BY-NC license

Nanoscale, 2019,11, 17357-17367

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Nanodiamond uptake in colon cancer cells: the influence of direction and trypsin-EDTA treatment

A. Sigaeva, A. Morita, S. R. Hemelaar and R. Schirhagl, Nanoscale, 2019, 11, 17357 DOI: 10.1039/C9NR04228H

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