Issue 24, 2009
From the journal:

Lab on a Chip

Rapid multicomponent optical proteinpatterning

Jonathan M. Bélisle,ab   Dario Kunikab  and  Santiago Costantino*abc  

* Corresponding authors

a Maisonneuve-Rosemont Hospital, University of Montreal, QC, Canada
E-mail: santiago.costantino@umontreal.ca

b Institute of Biomedical Engineering, University of Montreal, QC, Canada

c Department of Ophthalmology, University of Montreal, QC, Canada

Abstract

Cells sense spatial distributions of molecules which trigger signal transduction pathways that induce the cell to migrate or extend by remodelling the cytoskeleton. However, the influence of local and small variations of extracellular protein concentration on chemotaxis is not fully understood, due in part to the lack of simple and precise methods to pattern proteinsin vitro. We recently developed a new technology to fabricate such patterns which relies on photobleaching fluorophores to adsorb proteins on a cell culture substrate: laser-assisted protein adsorption by photobleaching (LAPAP). Here we report several key improvements to LAPAP: we created arbitrary patterns made of several different proteins simultaneously, we reduced the fabrication time more than one order of magnitude and we used secondary antibodies to significantly enlarge the spectrum of proteins that can be employed. As a result, multicomponent protein gradients can be produced using reagents that are typically available in life science research laboratories on a standard inverted microscope equipped with a camera port.

Graphical abstract: Rapid multicomponent optical protein patterning

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Article information

DOI
https://doi.org/10.1039/B911967A
Article type
Paper
Submitted
18 Jun 2009
Accepted
15 Sep 2009
First published
15 Oct 2009

Lab Chip, 2009,9, 3580-3585

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Rapid multicomponent optical protein patterning

J. M. Bélisle, D. Kunik and S. Costantino, Lab Chip, 2009, 9, 3580 DOI: 10.1039/B911967A

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