Issue 9, 2010
From the journal:

Molecular BioSystems

CDK2AP1/DOC-1 is a bona fide subunit of the Mi-2/NuRD complex†‡

Cornelia G. Spruijt,§a   Stefanie J. J. Bartels,§b   Arie B. Brinkman,b   Jorrit V. Tjeertes,b   Ina Poser,c   Hendrik G. Stunnenbergb  and  Michiel Vermeulen*a  

* Corresponding authors

a Department of Physiological Chemistry and Cancer Genomics Centre, University Medical Center Utrecht, Utrecht, The Netherlands
E-mail: M.Vermeulen-3@umcutrecht.nl

b Radboud University, Department of Molecular Biology, Nijmegen Centre for Molecular Life Sciences, Nijmegen, The Netherlands

c Max Planck Institute for Molecular Cell Biology and Genetics, Dresden, Germany

Abstract

The Mi-2/NuRD (NUcleosome Remodeling and histone Deacetylase) chromatin remodeling complex is a large heterogeneous multiprotein complex associated with transcriptional repression. Here we apply a SILAC based quantitative proteomics approach to show that all known Mi-2/NuRD complex subunits co-purify with Cyclin Dependent Kinase 2 Associated Protein1 (CDK2AP1), also known as Deleted in Oral Cancer 1 (DOC-1). DOC-1 displays in vitro binding affinity for methylated DNA as part of the meCpG binding MBD2/NuRD complex. In luciferase reporter assays, DOC-1 is a potent repressor of transcription. Finally, immunofluorescence experiments reveal co-localization between MBD2 and DOC-1 in mouse NIH-3T3 nuclei. Collectively, these results indicate that DOC-1 is a bona fide subunit of the Mi-2/NuRD chromatin remodeling complex.

Graphical abstract: CDK2AP1/DOC-1 is a bona fide subunit of the Mi-2/NuRD complex

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Article information

DOI
https://doi.org/10.1039/C004108D
Article type
Paper
Submitted
11 Mar 2010
Accepted
20 Apr 2010
First published
04 Jun 2010

Mol. BioSyst., 2010,6, 1700-1706

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CDK2AP1/DOC-1 is a bona fide subunit of the Mi-2/NuRD complex

C. G. Spruijt, S. J. J. Bartels, A. B. Brinkman, J. V. Tjeertes, I. Poser, H. G. Stunnenberg and M. Vermeulen, Mol. BioSyst., 2010, 6, 1700 DOI: 10.1039/C004108D

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