Issue 11, 2012
From the journal:

Molecular BioSystems

Thioflavin-S staining coupled to flow cytometry. A screening tool to detect in vivoprotein aggregation

Alba Espargaró,a   Raimon Sabate*bc  and  Salvador Ventura*ad  

* Corresponding authors

a Institut de Biotecnologia i Biomedicina, Universitat Autònoma de Barcelona, 08193-Bellaterra, Barcelona, Spain
E-mail: salvador.ventura@uab.es
Fax: +34-935811264
Tel: +34-935868147

b Departament de Fisicoquímica, Facultat de Farmàcia, Universitat de Barcelona, Avda. Joan XXIII s/n, E-08028-Barcelona, Spain
E-mail: rsabate@ub.edu
Fax: +34-93-4035987
Tel: +34-934035986

c Institut de Nanociència i Nanotecnologia (IN2UB), Spain

d Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, 08193-Bellaterra, Barcelona, Spain

Abstract

Amyloid deposits are associated with an increasing number of human disorders, including Alzheimer's and Parkinson's diseases. Recent studies provide compelling evidence for the existence of amyloid-like conformations in the insoluble bacterial inclusion bodies (IBs) produced during the recombinant expression of amyloidogenic proteins. This makes prokaryotic cells a physiologically relevant system to study the mechanisms of in vivo amyloid deposition. We show here that the application of flow cytometry to detect Thioflavin-S (Th-S) fluorescence provides a fast, robust, quantitative, non-invasive method to screen for the presence of in vivo intracellular amyloid-like aggregates in bacteria, with potential application in the analysis of the impact of genetic mutations or chemical compounds on the aggregation of disease-associated polypeptides.

Graphical abstract: Thioflavin-S staining coupled to flow cytometry. A screening tool to detect in vivo protein aggregation

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Article information

DOI
https://doi.org/10.1039/C2MB25214G
Article type
Method
Submitted
30 May 2012
Accepted
23 Jul 2012
First published
24 Jul 2012

Mol. BioSyst., 2012,8, 2839-2844

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Thioflavin-S staining coupled to flow cytometry. A screening tool to detect in vivo protein aggregation

A. Espargaró, R. Sabate and S. Ventura, Mol. BioSyst., 2012, 8, 2839 DOI: 10.1039/C2MB25214G

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