Self-assembled magnetic theranostic nanoparticles for highly sensitive MRI of minicircle DNA delivery

Qian Wan; Lisi Xie; Lin Gao; Zhiyong Wang; Xiang Nan; Hulong Lei; Xiaojing Long; Zhi-Ying Chen; Cheng-Yi He; Gang Liu; Xin Liu; Bensheng Qiu

doi:10.1039/C2NR32438E

Self-assembled magnetic theranostic nanoparticles for highly sensitive MRI of minicircle DNA delivery†

Qian Wan,‡^a Lisi Xie,‡^a Lin Gao,^a Zhiyong Wang,*^a Xiang Nan,^a Hulong Lei,^a Xiaojing Long,^a Zhi-Ying Chen,^b Cheng-Yi He,^b Gang Liu,^d Xin Liu^a and Bensheng Qiu*^ac

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* Corresponding authors

^a Paul C. Lauterbur Research Center for Biomedical Imaging, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China
E-mail: zy.wang@siat.ac.cn, bs.qiu@siat.ac.cn
Fax: +86-755-86392299
Tel: +86-755-86392268

^b Center for Gene and Cell Engineering, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China

^c Department of Radiology, University of Washington School of Medicine, USA

^d Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen, China

Abstract

As a versatile gene vector, minicircle DNA (mcDNA) has a great potential for gene therapy. However, some serious challenges remain, such as to effectively deliver mcDNA into targeted cells/tissues and to non-invasively monitor the delivery of the mcDNA. Superparamagnetic iron oxide (SPIO) nanoparticles have been extensively used for both drug/gene delivery and diagnosis. In this study, an MRI visible gene delivery system was developed with a core of SPIO nanocrystals and a shell of biodegradable stearic acid-modified low molecular weight polyethyleneimine (Stearic–LWPEI) via self-assembly. The Stearic–LWPEI–SPIO nanoparticles possess a controlled clustering structure, narrow size distribution and ultrasensitive imaging capacity. Furthermore, the nanoparticle can effectively bind with mcDNA and protect it from enzymatic degradation. In conclusion, the nanoparticle shows synergistic advantages in the effective transfection of mcDNA and non-invasive MRI of gene delivery.

Nanoscale

Self-assembled magnetic theranostic nanoparticles for highly sensitive MRI of minicircle DNA delivery†

Abstract

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Self-assembled magnetic theranostic nanoparticles for highly sensitive MRI of minicircle DNA delivery

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