Issue 26, 2014
From the journal:

Chemical Communications

Profiling of RNA modifications by multiplexed stable isotope labelling†

Stefanie Kellner,a   Jennifer Neumann,a   David Rosenkranz,b   Svetlana Lebedeva,c   René F. Ketting,c   Hans Zischler,b   Dirk Schneidera  and  Mark Helm*a  

* Corresponding authors

a Institute of Pharmacy and Biochemistry, Johannes Gutenberg University, 5 Staudinger Weg, Mainz, Germany
E-mail: mhelm@uni-mainz.de
Fax: +49 6131 39 20373
Tel: +49 6131 39 25731

b Institute for Anthropology Johannes Gutenberg University Mainz, Anselm-Franz-von-Bentzel-Weg 7, D-55128 Mainz, Germany

c Institute of Molecular Biology Mainz, Ackermannweg 4, D-55128 Mainz, Germany

Abstract

The combination of 15N/13C stable isotope labelling (SIL) and LC-MS/MS revealed a total of 52 modifications in RNA from E. coli and yeast, including 10 previously undescribed modifications. Two modifications, N-ribosylnicotinamide and 2-methylthioadenosine, were newly detected in species hitherto thought not to contain these modifications.

Graphical abstract: Profiling of RNA modifications by multiplexed stable isotope labelling

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C3CC49114E
Article type
Communication
Submitted
29 Nov 2013
Accepted
05 Feb 2014
First published
24 Feb 2014

Chem. Commun., 2014,50, 3516-3518

Permissions

Request permissions

Profiling of RNA modifications by multiplexed stable isotope labelling

S. Kellner, J. Neumann, D. Rosenkranz, S. Lebedeva, R. F. Ketting, H. Zischler, D. Schneider and M. Helm, Chem. Commun., 2014, 50, 3516 DOI: 10.1039/C3CC49114E

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements