Interleukin-6 Expression After Repeated Bouts of Eccentric Exercise

 

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Abstract

Plasma interleukin-6 (IL-6) is known to increase in response to eccentric exercise due to an acute-phase immune response. However, the severity of muscle injury is reduced with repeated bouts of eccentric exercise, possibly as a result of decreases in plasma IL-6. This study determined the response of IL-6 mRNA and IL-6, troponin-I (sTnI), muscle strength, and soreness as a result of repeated bouts of eccentric exercise. Eight males underwent two eccentric exercise bouts (3 wk apart) involving 7 sets of 10 repetitions at 150 % of the isotonic 1-RM of the dominant knee extensors. Blood samples were taken before, after and 2, 4, 6, 24, 48 and 96 h post-exercise. Strength and soreness ratings were assessed before and at 24, 48 and 96 h-post. Data were analyzed with 2 × 4 and 2 × 8 ANOVAs and the non-parametric Friedman test (p < 0.05). Both IL-6 mRNA and IL-6 underwent peak increases (p < 0.05) at 4 h-post and 6 h-post, respectively, but were not different between bouts. However, there were significant changes (p < 0.05) in sTnI, strength, and soreness that were greater after the first bout than the second, characteristic of the repeated bout effect. These results indicate that changes in sTnI, strength and soreness were less with the second eccentric exercise bout whereas the changes in both IL-6 mRNA and protein were not effected between bouts.

References

• 1 Aki T, Yanagisawa S, Akanuma H. Identification and characterization of positive regulatory elements in the human glyceraldehyde 3-phosphate dehydrogenase gene promoter.  J Biochem. 1997;  122 217-228
  PubMedGoogle Scholar
• 2 Brown S, Child R, Day S, Donelly A. Indices of skeletal muscle damage and connective tissue breakdown following eccentric muscle contractions.  Eur J Appl Physiol. 1997;  75 369-374
  CrossrefPubMedGoogle Scholar
• 3 Bruunsgaard H, Galbo H, Halkjaer-Kristensen J, Johansen T, MacLean D, Pedersen B. Exercise-induced increase in serum interleukin-6 in humans is related to muscle damage.  J Physiol. 1997;  499.3 833-841
  PubMedGoogle Scholar
• 4 Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidium thiocynate-phenol-chloroform extraction.  Anal Biochem. 1987;  162 156-159
  PubMedGoogle Scholar
• 5 Croisier J, Camus G, Venneman I, Deby-Dupont G, Juchmes-Ferir A, Lamy M, Crielaard J, Deby C, Duchateau J. Effects of training on exercise-induced muscle damage and interleukin-6 production.  Muscle Nerve. 1999;  22 208-212
  PubMedGoogle Scholar
• 6 Ausubel F, Brent R, Kingston R, Moore D, Seidman J, Smith J, Struhl K. Current Protocols in Molecular Biology.  Short Protocols in Molecular Biology. New York, NY; Wiley and Sons 1999: pp. A3 - 10-A3 - 11
  Google Scholar
• 7 Ebisui C, Tsujinaka T, Morimoto T, Kan K, Iijima S, Yano M, Kominami E, Tanaka K, Monden M. Interleukin-6 induces proteolysis by activating intracellular proteases (cathepsons B and L, proteasome) in C2C12 myotubes.  Clin Sci. 1995;  89 431-439
  PubMedGoogle Scholar
• 8 Goodman M. Interleukin-6 induces skeletal muscle protein breakdown in rats.  Proc Soc Exp Biol Med. 1994;  205 182-185
  PubMedGoogle Scholar
• 9 Hortobagyi T, Houmard J, Fraser D, Dudek R, Lambert J, Tracy J. Normal forces and myofibrillar disruption after repeated eccentric exercise.  J Appl Physiol. 1998;  84 492-498
  PubMedGoogle Scholar
• 10 Hyatt J, Clarkson P. Creatine kinase release and clearance using MM variants following repeated bouts of eccentric exercise.  Med Sci Sports Exerc. 1998;  30 1059-1065
  PubMedGoogle Scholar
• 11 Jonsdottir I, Schjerling P, Ostrowski K, Asp S, Richter E. Muscle contractions induce interleukin-6 mRNA production in rat skeletal muscles.  J Physiol. 2000;  528 157-163
  CrossrefPubMedGoogle Scholar
• 12 Kayashima S, Ohono H, Fujioka T, Taniguchi N, Nagata N. Leucocytosis as a marker of organ damage induced by chronic strenuous physical exercise.  Eur J Appl Physiol. 1995;  70 413-420
  PubMedGoogle Scholar
• 13 Kraemer W, Fry A. Strength testing: development and evaluation of methodology. In: Maud P, Foster C (eds.) Physiological Assessment of Human Fitness. Champaign, IL; Human Kinetics 1995: pp. 115-133
  Google Scholar
• 14 Mair J, Koller A, Artner-Dworzak E, Haid C, Wicke K, Judmaier W, Puschendorf B. Effects of exercise on plasma myosin heavy chain fragments and MRI of skeletal muscle.  J Appl Physiol. 1992;  72 656-663
  PubMedGoogle Scholar
• 15 Malm , C , Nyberg P, Engstrom M, Sjodin B, Lenkei R, Ekblom B, Lundberg I. Immunological changes in human skeletal muscle and blood after eccentric exercise and multiple biopsies.  J Physiol. 2000;  529 243-262
  CrossrefPubMedGoogle Scholar
• 16 Mayhew T, Rothstein J, Finucane S, Lamb R. Muscular adaptation to concentric and eccentric exercise at equal power levels.  Med Sci Sports Exerc. 1995;  27 868-873
  PubMedGoogle Scholar
• 17 McHugh M, Connolly D, Eston R, Geim G. Exercise-induced muscle damage and potential mechanisms for the repeated bout effect.  Sports Med. 1999;  27 157-170
  CrossrefPubMedGoogle Scholar
• 18 Moldoveanu A, Shephard R, Shek P. Exercise elevates plasma levels but not gene expression of IL-1β, IL-6, and TNF-α in blood mononuclear cells.  J Appl Physiol. 2000;  89 1499-1504
  PubMedGoogle Scholar
• 19 Nosaka K, Clarkson P. Muscle damage following repeated bouts of high force eccentric exercise.  Med Sci Sports Exerc. 1995;  27 1263-1269
  PubMedGoogle Scholar
• 20 Ostrowski K, Hermann C, Bangash A, Schjerling P, Nielsen J, Pedersen B. A trauma-like elevation of plasma cytokines in humans in response to treadmill running.  J Physiol. 1998;  513 889-894
  CrossrefPubMedGoogle Scholar
• 21 Ostrowski K, Rhode T, Zacho M. Asp S, Pedersen B. Evidence that interleukin-6 is produced in human skeletal muscle during prolonged running.  J Physiol. 1998;  508 949-953
  CrossrefPubMedGoogle Scholar
• 22 Pedersen B, Ostrowski K, Rhode T, Bruunsgaard H. The cytokine response to exercise.  Can J Physiol Pharmacol. 1998;  76 505-511
  CrossrefPubMedGoogle Scholar
• 23 Puntschart A, Claassen H, Jostarndt K, Hoppeler H, Billeter R. mRNAs of enzymes involved in energy metabolism and mtDNA are increased in endurance-trained athletes.  Am J Physiol. 1995;  269 C619-625
  PubMedGoogle Scholar
• 24 Rhode T, MacLean D, Richter E, Kiens B, Pedersen B. Prolonged submaximal eccentric exercise is associated with increased levels of plasma IL-6.  Am J Physiol. 1997;  273 E85-E91
  PubMedGoogle Scholar
• 25 Sorichter S, Mair J, Koller A, Gebert W, Rama D, Calzolari C, Artner-Dworzak E, Puschendorf B. Skeletal troponin I as a marker of exercise-induced muscle damage.  J Appl Physiol. 1997;  83 1076-1082
  PubMedGoogle Scholar
• 26 Sorichter S, Mair J, Koller A, Secnik P, Parrak V, Haid C, Muller E, Puschendorf B. Muscular adaptation and strength during the early phase of eccentric training: influence of training frequency.  Med Sci Sports Exerc. 1997;  29 1646-1652
  PubMedGoogle Scholar
• 27 Steensberg A, van Hall G, Osada T, Sacchetti M, Saltin B, Pedersen B. Production of interleukin-6 in contracting human skeletal muscles can account for the exercise-induced increases in plasma interleukin-6.  J Physiol. 2000;  529 237-242
  CrossrefPubMedGoogle Scholar
• 28 Willoughby D, Pelsue S. Muscle strength and qualitative myosin heavy chain isoform mRNA expression after moderate-and high-intensity weight training in the elderly.  J Aging Phys Act. 1998;  6 327-339
  PubMedGoogle Scholar
• 29 Willoughby D, Pelsue S. Effects of high-intensity strength training on steady-state myosin heavy chain mRNA expression.  J Exer Physiol. 2000;  3 13-25
  PubMedGoogle Scholar
• 30 Willoughby D, Priest J, Jennings R. Myosin heavy chain isoform and ubiquitin protease mRNA expression after passive leg cycling in individuals with spinal cord injury.  Arch Phys Med Rehab. 2000;  81 157-163
  PubMedGoogle Scholar
• 31 Willoughby D, Rosene J. Effects of oral creatine and heavy resistance training on myosin heavy chain expression.  Med Sci Sports Exerc. 2001;  33 1674-1681
  PubMedGoogle Scholar

D. S. Willoughby, Ph. D.

Molecular Kinesiology Laboratory · Department of Kinesiology · Texas Christian University

TCU Box 297730 · Fort Worth, TX 76129 · USA ·

Phone: +1 (817) 257 7665

Fax: +1 (817) 257 7702

Email: d.willoughby@tcu.edu