Most genes encoding cytoplasmic intermediate filament (IF) proteins of the nematode Caenorhabditis elegans are required in late embryogenesis
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BBLN-1 is essential for intermediate filament organization and apical membrane morphology
2021, Current BiologyCitation Excerpt :We next determined whether the apical localization of BBLN-1 depends on IFs. We used RNAi feeding to knock down each of the six intestinal IF proteins that are part of the endotube.10,19,31,32 We observed significant reduction in apical BBLN-1 upon knockdown of IFB-2, IFD-1, IFD-2, or IFP-1 (Figures 3A and 3B).
Analysis of the novel excretory cell expressed ECP-1 protein and its proposed ECP-1/IFC-2 fusion protein EXC-2 in the nematode Caenorhabditis elegans
2019, Gene Expression PatternsCitation Excerpt :The primary (but not sole) function of the cytoplasmic IFs is to provide resistance against mechanical stress. This view is supported by a variety of epidermal fragility syndromes of mutated human keratins (Irvine and McLean, 1999), by knock-out experiments in mice (i.e. Hesse et al., 2000; Vijayaraj et al., 2009), as well as by (reverse) genetics (RNAi) in Caenorhabditis elegans (C. elegans; Karabinos et al., 2001, 2003, 2004; Hapiak et al., 2003; Woo et al., 2004; Hüsken et al., 2008; Zhang et al., 2011; Geisler et al., 2016). The nematode C. elegans has a single and essential nuclear lamin (LMN-1; Liu et al., 2000; Fraser et al., 2000), and eleven cytoplasmic IF genes (ifa-1 to ifa-4, ifb-1, ifb-2, ifc-1, ifc-2, ifd-1, ifd-2, ifp-1), some of which give rise to alternatively spliced variants (Dodemont et al., 1994; Karabinos et al., 2001, 2003; Woo et al., 2004).
Intermediate filament (IF) proteins IFA-1 and IFB-1 represent a basic heteropolymeric IF cytoskeleton of nematodes: A molecular phylogeny of nematode IFs
2019, GeneCitation Excerpt :As expected, all newly identified sequences have a long rod domain (LRD; Table 1 and Fig. S1K) typical for cytoplasmic IF proteins of invertebrates and, except IFC and IFD-2, have also the lamin homology domain (LHD) in their tails (Table 1 and Fig. S1; for review on invertebrate cytoplasmic IFs see Erber et al., 1998). Moreover, the newly identified IFB-1, IFB-2, IFC-1, IFC-2, IFD-2 and IFP-1 protein sequences harbour several previously established unique rod deletions in their corresponding C. elegans counterparts (Fig. S1K; for details see Dodemont et al., 1994; Karabinos et al., 2003a, 2004), which trace them to the common IFB-1, IFB-2, IFC-1, IFC-2, IFD-2 and IFP-1 ancestor, respectively. The same holds, also, for the newly established IFCDP-1 and IFCDP-2 IF sequences, which share with the IFC, IFD and/or IFP-1 proteins the unique deletion in the L12 linker and the coil 1b rod domain (Fig. S1K).
Intermediate Filaments in Caenorhabditis elegans
2016, Methods in EnzymologyMethods in Cell Biology: Analysis of Cell Polarity in C. elegans Embryos
2012, Methods in Cell Biology