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Distribution of regulator of G protein signaling 8 (RGS8) protein in the cerebellum

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Abstract

The regulator of G protein signaling (RGS) proteins modulate heterotrimeric G protein signaling. RGS8 was identified as a brainspecific RGS protein of 180 amino acids. Biochemical studies indicated that RGS8 binds to Gao and Gαi3, and that it functions as a GTPase-activating protein (GAP) for Gα subunits. Physiological investigations demonstrated that RGS8 is not a simple negative regulator, but accelerates the G-protein-coupled responses. In situ hybridization analysis showed a highly dense expression of RGS8 mRNA in Purkinje cells of the cerebellum in rat brain. When the cellular distribution of RGS8 was examined in non-neural cells transfected with RGS8 cDNA, the protein was found to be concentrated in nuclei. Further, co-expression of constitutively active Gao resulted in the translocation of RGS8 protein to the plasma membrane. The cellular distribution of the RGS8 protein in cerebellar Pukinje cells was also studied in detail. It was shown that the protein is excluded from the nuclei and distributed in the cell body and dendrites except the axons of Purkinje cells. Thus, it is evident that there is a novel mechanism controlling the distribution of RGS8 protein in cerebellar Purkinje cells.

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Authors and Affiliations

  1. Department of Molecular Cell Signaling, Tokyo Metropolitan Institute for Neuroscience, 2-6 Musashidai, Fuchu-shi, 183-8526, Tokyo, Japan

    Osamu Saitoh PhD, Ikuo Masuho, Masayuki Itoh, Keiichiro Komori & Megumi Odagiri

  2. Graduate School of Science and Technology, Chiba University, Chiba, Japan

    Ikuo Masuho

  3. Department of Biomolecular Science, Faculty of Science, Toho University, Chiba, Japan

    Masayuki Itoh

  4. Department of Physiology, Tokyo Medical and Dental University, Graduate School and Faculty of Medicine, Yushima, Tokyo, Japan

    Hideki Abe

  5. Department of Applied Chemistry, Faculty of Engineering, Kanagawa Institute of Technology, Kanagawa, Japan

    Keiichiro Komori

Authors
  1. Osamu Saitoh PhD
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  2. Ikuo Masuho
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  3. Masayuki Itoh
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  4. Hideki Abe
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  5. Keiichiro Komori
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  6. Megumi Odagiri
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Correspondence to Osamu Saitoh PhD.

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Saitoh, O., Masuho, I., Itoh, M. et al. Distribution of regulator of G protein signaling 8 (RGS8) protein in the cerebellum. Cerebellum 2, 154–160 (2003). https://doi.org/10.1080/14734220309409

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  • DOI: https://doi.org/10.1080/14734220309409

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