1887

Abstract

Summary: The conditions for the formation, the isolation and the purification of a new class of disaccharide derivatives, namely 3-ketoglycosides, are described. These compounds are formed by the action of certain bacteria, provisionally called , on lactose, maltose, lacto- or maltobionate. From lactose about 76 % of the total amount was converted into the corresponding 3-ketoglyco-side. Neither the corresponding monoses, gluconate nor equimolar mixtures of these substances were oxidized this way. The new substances were ultimately purified by paper chromatography. No crystalline preparations have so far been obtained. These bacteria contain strong hydrolases for either maltose or lactose, and weak ones for the bionates. The yield of 3-ketoglycosides is inversely proportional to the hydrolase activity.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-22-1-129
1960-02-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/22/1/mic-22-1-129.html?itemId=/content/journal/micro/10.1099/00221287-22-1-129&mimeType=html&fmt=ahah

References

  1. Abdel-Akher M., Smith F. 1951; The detection of carbohydrate esters and lactones after separation by paper chromatography. J. Amer. chem. Soc 73:5859
    [Google Scholar]
  2. Bernaerts M.J., De Ley J. 1957; The catabolism of lactose and lactobionate by Corynebacterium simplex. . Leeuwenhoek, ned. Tijdschr 23:333
    [Google Scholar]
  3. Bernaerts M.J., De Ley J. 1958; 3-Ketoglycosides, new intermediates in the bacterial catabolism of disaccharides. Biochim. biophys.Acta 30:661
    [Google Scholar]
  4. Eddy B.P. 1958; bacterial oxidation of lactose and melibiose. Nature; London: 181904
    [Google Scholar]
  5. Helferich B., Pigman W., Isbell H.S. 1939; Die Spaltbarkeit von Lacto-bionsäure durch Süssmandel-emulsin. . Z. physiol. Chem 261:189
    [Google Scholar]
  6. Isbell H.S., Frush H.L. 1931; The oxidation of sugars. 1. The electrolytic oxidation of aldoses in the presence of a bromide and calcium carbonate. J. Res. not. Bur. Stand 6:1145
    [Google Scholar]
  7. Isbell H.S., Pigman W., Frush H.L. 1940; Reducing powers of various sugars with alkaline copper-citrate reagent. J. Res. not. Bur. Stand 24:241
    [Google Scholar]
  8. Kluyver A.J., Custers M. 1940; The suitability of disaccharides as respiration and assimilation substrates for yeasts which do not ferment sugars. Leeuwenhoek ned. Tijdschr 6:121
    [Google Scholar]
  9. Kluyver A.J., De Ley J., Rijven A. 1951; The formation and consumption of lactobionic and maltobionic acids by Pseudomonas species. Leeuwenhoek ned. Tijdschr 17:1
    [Google Scholar]
  10. Lanning M.C., Cohen S.S. 1951; The detection and estimation of 2-ketohexonic acids. J. biol. Chem 189:109
    [Google Scholar]
  11. Lindberg B., Theander O. 1954; The oxidation of glycosides. IV. Oxidation of methyl-β-d-glucoside with dichxomate. Acta chem.scand 8:1870
    [Google Scholar]
  12. Pigman W. 1944; Specificity, classification, and mechanism of action of the glycosidases. Advanc. Enzymol 4:41
    [Google Scholar]
  13. Stodoea F.H., Lockwood L.B. 1947; The oxidation of lactose and maltose to bionic acids by Pseudomonas. . J. biol. Chem 171:213
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-22-1-129
Loading
/content/journal/micro/10.1099/00221287-22-1-129
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error