1887

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Volume 140, Issue 1

K12 regains its O antigen Free

Abstract

Summary: Extant K12 strains are phenotypically rough, their lipopolysaccharide having a complete core structure, but no O antigen. We used DNA hybridization and DNA sequencing to show that the rough phenotype of this strain is due to the presence of one of two independent mutations in the gene cluster. The mutation, consisting of an IS5 insertion at the downstream end of , is present in strain EMG2, which is representative of most K12 derivatives. The mutation is a deletion at the upstream end of , and was found in strain WG1. A gene cloned from strain WG1 could complement the mutation in strain EMG2, and the complemented strain produced O antigen which was typed as O16 with cross reaction to O17.

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Keyword(s): Escherichia coli K12 , lipopolysaccharide , O antigen and rfb gene mutations
© Society for General Microbiology 1994
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1994-01-01
2024-04-25
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Escherichia coli K12 regains its O antigen
Microbiology 140, 49 (1994); https://doi.org/10.1099/13500872-140-1-49
/content/journal/micro/10.1099/13500872-140-1-49
/content/journal/micro/10.1099/13500872-140-1-49
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