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Volume 165, Issue 8

Utilization of larvae to characterize the development of infection Free

Abstract

is a human opportunistic pathogen that causes a wide range of superficial and systemic infections in susceptible patients. Here we describe how an inoculum of activates the cellular and humoral response of larvae while growing and disseminating throughout the host, forming nodules and ultimately killing the host. An inoculum of (2×10 larva) decreased larval viability at 24 (80±5.77 %), 48 (55.93±5.55 %) and 72 h (10.23±2.97 %) and was accompanied by significant proliferation and dissemination of between 6 and 48 h and the formation of nodules in the host. The hemocyte (immune cell) densities increased between 4 and 24 h and hemocytes isolated from larvae after 24 h exposure to heat-killed (2×10 larva) showed altered killing kinetics as compared to those from control larvae. Alterations in the humoral immune response of larvae 6 and 24 h post-infection were also determined by quantitative shotgun proteomics. The proteome of 6 h-infected larvae was enriched for antimicrobial proteins, proteins of the prophenoloxidase cascade and a range of peptidoglycan recognition proteins. By 24 h there was a significant increase in the abundance of a range of antimicrobial peptides with anti-staphylococcal activity and proteins associated with nodule formation. The results presented here indicate how interacts with the larval immune response, induces the expression of a variety of immune-related peptides and also forms nodules which are a hallmark of soft tissue infections during human infection.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Galleria , immunity , infection , mini-model and Staphylococcus
© 2019 The Authors
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2019-08-01
2024-04-19
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Utilization of Galleria mellonella larvae to characterize the development of Staphylococcus aureus infection
Microbiology 165, 863 (2019); https://doi.org/10.1099/mic.0.000813
/content/journal/micro/10.1099/mic.0.000813
/content/journal/micro/10.1099/mic.0.000813
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