1887

Abstract

Human small and large intestinal tissue was used to study the interaction of with its target tissue. The strain used for the study was 81-176 (+pVir). Tissue was processed for scanning and transmission electron microscopy, and by immunohistochemistry for light microscopy. Organisms adhered to the apical surface of ileal tissues at all time points in large numbers, in areas where mucus was present and in distinct groups. Microcolony formation was evident at 1–2 h, with bacteria adhering to mucus on the tissue surface and to each other by flagellar interaction. At later time points (3–4 h), biofilm formation on ileal tissue was evident. Flagellar mutants did not form microcolonies or biofilms in tissue. Few organisms were observed in colonic tissue, with organisms present but not as abundant as in the ileal tissue. This study shows that 81-176 can form microcolonies and biofilms on human intestinal tissue and that this may be an essential step in its ability to cause diarrhoea in man.

Loading

Article metrics loading...

/content/journal/micro/10.1099/mic.0.039867-0
2010-10-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/156/10/3079.html?itemId=/content/journal/micro/10.1099/mic.0.039867-0&mimeType=html&fmt=ahah

References

  1. Asakura H., Yamasaki M., Yamamoto S., Igimi S. 2007; Deletion of peb4 gene impairs cell adhesion and biofilm formation in Campylobacter jejuni. FEMS Microbiol Lett 275:278–285
    [Google Scholar]
  2. Black R. E., Levine M. M., Clements M. L., Hughes T. P., Blaser M. J. 1988; Experimental Campylobacter jejuni infection in humans. J Infect Dis 157:472–479
    [Google Scholar]
  3. Butzler J. P. 1981; New prospects for treatment and prevention. In Acute Enteric Infections in Children p 63 Edited by Holme T., Holmgren J., Merson M., Mollby R. Amsterdam: Elsevier;
    [Google Scholar]
  4. Golden N. J., Acheson D. W. K. 2002; Identification of motility and autoagglutination of Campylobacter jejuni mutants by random transposon mutagenesis. Infect Immun 70:1761–1771
    [Google Scholar]
  5. Grant A. J., Woodward J., Maskell D. J. 2006; Development of an ex vivo organ culture model using human gastro-intestinal tissue and Campylobacter jejuni. FEMS Microbiol Lett 263:240–243
    [Google Scholar]
  6. Guerry P. 2007; Campylobacter flagella: not just for motility. Trends Microbiol 15:456–461
    [Google Scholar]
  7. Hanning I., Jarquin R., Slavik M. 2008; Campylobacter jejuni as a secondary colonizer of poultry biofilms. J Appl Microbiol 105:1199–1208
    [Google Scholar]
  8. Hanning I., Donoghue D. J., Jarquin R., Kumar G. S., Aguiar V. F., Metcalf J. H., Reyes-Herrera I., Slavik M. 2009; Campylobacter biofilm phenotype exhibits reduced colonization potential in young chickens and altered in vitro virulence. Poult Sci 88:1102–1107
    [Google Scholar]
  9. Howard S. L., Jagannathan A., Soo E. C., Hui J. P., Aubry A. J., Ahmed I., Karlyshev A., Kelly J. F., Jones M. A. other authors 2009; Campylobacter jejuni glycosylation island important in cell charge, legionaminic acid biosynthesis, and colonization of chickens. Infect Immun 77:2544–2556
    [Google Scholar]
  10. Hu L., Tall B. D., Curtis S. K., Kopecko D. J. 2008; Enhanced microscopic definition of Campylobacter jejuni 81-176 adherence to, invasion of, translocation across, and exocytosis from polarized human intestinal Caco-2 cells. Infect Immun 76:5294–5304
    [Google Scholar]
  11. Kalmokoff M., Lanthier P., Tremblay T. L., Foss M., Lau P. C., Sanders G., Austin J., Kelly J., Szymanski C. M. 2006; Proteomic analysis of Campylobacter jejuni 11168 biofilms reveals a role for the motility complex in biofilm formation. J Bacteriol 188:4312–4320
    [Google Scholar]
  12. Konkel M. E., Monteville M. R., Rivera-Amill V., Joens L. A. 2001; The pathogenesis of Campylobacter jejuni-mediated enteritis. Curr Issues Intest Microbiol 2:55–71
    [Google Scholar]
  13. Krause-Gruszczynska M., Rohde M., Hartig R., Genth H., Schmidt G., Keo T., König W., Miller W. G., Konkel M. E., Backert S. 2007; Role of the small Rho GTPases Rac1 and Cdc42 in host cell invasion of Campylobacter jejuni. Cell Microbiol 9:2431–2444
    [Google Scholar]
  14. Skirrow M., Blaser M. 2000; Clinical aspects of Campylobacter infection. In Campylobacter pp 68–88 Edited by Nachamkin I., Blaser M. J. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  15. Svensson S. L., Frirdich E., Gaynor E. 2008; Survival strategies of Campylobacter jejuni: stress responses, the viable but nonculturable state and biofilms. In Campylobacter, 3rd edn. pp 571–590 Edited by Nachamkin I., Szymanski C., Blaser M. J. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  16. Svensson S. L., Davis L. M., MacKichan J. K., Allan B. J., Pajaniappan M., Thompson S. A., Gaynor E. C. 2009; The CprS sensor kinase of the zoonotic pathogen Campylobacter jejuni influences biofilm formation and is required for optimal chick colonization. Mol Microbiol 71:253–272
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/mic.0.039867-0
Loading
/content/journal/micro/10.1099/mic.0.039867-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error