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Volume 160, Issue 8

Editor's Choice Identification of a ancillary copper detoxification mechanism by a comparative analysis of the genome-wide transcriptional response to copper and zinc excess Free

Abstract

Copper and zinc are essential metal ions, but toxic in excess. Bacteria have evolved different strategies to control their intracellular concentrations, ensuring proper supply while avoiding toxicity, including the induction of metal-specific as well as non-specific mechanisms. We compared the transcriptional profiles of Typhimurium after exposure to either copper or zinc ions in both rich and minimal media. Besides metal-specific regulatory networks many global stress-response pathways react to an excess of either of these metal ions. Copper excess affects both zinc and iron homeostasis by inducing transcription of these metal-specific regulons. In addition to the control of zinc-specific regulons, zinc excess affects the Cpx regulon and the σ envelope-stress responses. Finally, novel metal-specific upregulated genes were detected including a new copper-detoxification pathway that involves the siderophore enterobactin and the outer-membrane protein TolC. This work sheds light onto the transcriptional landscape of after copper or zinc overload, and discloses a new mechanism of copper detoxification.

  • Received:
  • Accepted:
  • Published Online:
Funding
This study was supported by the:
  • Agencia Nacional de Promoción Científica y Tecnológica
  • National Scientific and Technical Research Council (CONICET)
  • CONICET
  • ASM
  • A.V.E program
  • U.N.R.
  • NIH (Award AI083646, AI077645,, AI075093, AI073971, AI052237 and AI039557)
  • NIH (Award HHSN272200900040C)
  • USDA (Award 2011-67017-30127 and 2009-03579)
  • Binational Agricultural Research and Development Fund
  • Center for Produce Safety
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2014-08-01
2024-04-18
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Identification of a Salmonella ancillary copper detoxification mechanism by a comparative analysis of the genome-wide transcriptional response to copper and zinc excess
Microbiology 160, 1659 (2014); https://doi.org/10.1099/mic.0.080473-0
/content/journal/micro/10.1099/mic.0.080473-0
/content/journal/micro/10.1099/mic.0.080473-0
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