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Volume 160, Issue 11

Deletion of the sp. PCC 6803 gene cluster causes impaired cell growth under light–dark conditions Free

Abstract

In contrast to PCC 7942, few data exist on the timing mechanism of the widely used cyanobacterium sp. PCC 6803. The standard operon present in this organism was shown to encode a functional KaiC protein that interacted with KaiA, similar to the PCC 7942 clock. Inactivation of this operon in sp. PCC 6803 resulted in a mutant with a strong growth defect when grown under light–dark cycles, which was even more pronounced when glucose was added to the growth medium. In addition, mutants showed a bleaching phenotype. No effects were detected in mutant cells grown under constant light. Microarray experiments performed with cells grown for 1 day under a light–dark cycle revealed many differentially regulated genes with known functions in the ΔABC mutant in comparison with the WT. We identified the genes encoding the cyanobacterial phytochrome Cph1 and the light-repressed protein LrtA as well as several hypothetical ORFs with a complete inverse behaviour in the light cycle. These transcripts showed a stronger accumulation in the light but a weaker accumulation in the dark in Δ cells in comparison with the WT. In general, we found a considerable overlap with microarray data obtained for and mutants. These genes are known to be important regulators of cell metabolism in the dark. Strikingly, deletion of the ΔABC operon led to a much stronger phenotype under light–dark cycles in sp. PCC 6803 than in sp. PCC 7942.

  • Received:
  • Accepted:
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Funding
This study was supported by the:
  • German Research Fund (DFG) (Award WI 2014/5-1)
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2014-11-01
2024-04-25
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Deletion of the Synechocystis sp. PCC 6803 kaiAB1C1 gene cluster causes impaired cell growth under light–dark conditions
Microbiology 160, 2538 (2014); https://doi.org/10.1099/mic.0.081695-0
/content/journal/micro/10.1099/mic.0.081695-0
/content/journal/micro/10.1099/mic.0.081695-0
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