1887

Abstract

Enterovirus 71 (EV71) infection causes a myriad of diseases from mild hand-foot-and-mouth disease or herpangina to fatal meningoencephalitis complicated with neurogenic pulmonary oedema. Its pathogenesis, especially the CNS involvement, is not clearly understood. The aim of this study was to set up a mouse EV71 infection model with CNS involvement. EV71 virus was administrated orally to neonatal mice. The EV71-infected mice manifested a skin rash at an early stage and hind limb paralysis or death at a later stage. Immunohistochemical staining and virus isolation demonstrated that EV71 replicated in the small intestine, induced viraemia and spread to various organs. Kinetic studies showed that EV71 antigen was first detected in the intestine at 6 h, in the thoracic spinal cord at 24 h, in the cervical spinal cord at 50 h and in the brain stem at 78 h post-infection. Leukocyte infiltration was evident in the spinal cord and brain stem. Furthermore, EV71 virus could be transmitted to littermates within the same cage.

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2004-01-01
2024-04-19
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References

  1. Baumler A. J., Tsolis R. M., Bowe F. A., Kusters J. G., Hoffmann S., Heffron F. 1996; The pef fimbrial operon of Salmonella typhimurium mediates adhesion to murine small intestine and is necessary for fluid accumulation in the infant mouse. Infect Immun 64:61–68
    [Google Scholar]
  2. Crotty S., Hix L., Sigal L. J., Andino R. 2002; Poliovirus pathogenesis in a new poliovirus receptor transgenic mouse model: age-dependent paralysis and a mucosal route of infection. J Gen Virol 83:1707–1720
    [Google Scholar]
  3. Ho M. 2000; Enterovirus 71: the virus, its infections and outbreaks. J Microbiol Immunol Infect 33:205–216
    [Google Scholar]
  4. Ho M., Chen E. R., Hsu K. H., Twu S. J., Chen K. T., Tsai S. F., Wang J. R., Shih S. R. 1999; An epidemic of enterovirus 71 infection in Taiwan. N Engl J Med 341:929–935
    [Google Scholar]
  5. Huang C. C., Liu C. C., Chang Y. C., Chen C. Y., Wang S. T., Yeh T. F. 1999; Neurological complications in children with enterovirus 71 infection. N Engl J Med 341:936–942
    [Google Scholar]
  6. Liu C. C., Tseng H. W., Wang S. M., Wang J. R., Su I. J. 2000; An outbreak of enterovirus 71 infection in Taiwan, 1998: epidemiologic and clinical manifestations. J Clin Virol 17:23–30
    [Google Scholar]
  7. Lum L. C. S., Wong K. T., Lam S. K. 7 other authors 1998; Fatal enterovirus 71 encephalomyelitis. J Pediatr 133:795–798
    [Google Scholar]
  8. McMinn P. C. 2002; An overview of the evolution of enterovirus 71 and its clinical and public health significance. FEMS Microbiol Rev 26:91–107
    [Google Scholar]
  9. Mann M. A., Knipe D. M., Fischbach G. D., Fields B. N. 2002; Type 3 reovirus neuroinvasion after intramuscular inoculation: direct invasion of nerve terminals and age-dependent pathogenesis. Virology 303:222–231
    [Google Scholar]
  10. Minstry of Health The Executive Yuan. Taiwan, ROC: 1998; Deaths among children during an outbreak of hand, foot, and mouth disease – Taiwan, Republic of China, April–July 1998. Morb Mortal Wkly Rep 47:629–632
    [Google Scholar]
  11. Morrison L. A., Sidman R. L., Fields B. N. 1991; Direct spread of reovirus from the intestinal lumen to the central nervous system through vagal autonomic nerve fibers. Proc Natl Acad Sci U S A 88:3852–3856
    [Google Scholar]
  12. Ohka S., Nomoto A. 2001; Recent insights into poliovirus pathogenesis. Trends Microbiol 9:501–506
    [Google Scholar]
  13. Ren R., Racaniello V. R. 1992; Poliovirus spreads from muscle to the central nervous system by neural pathways. J Infect Dis 166:747–752
    [Google Scholar]
  14. Schmidt N. J., Lennett E. H., Ho H. H. 1974; An apparently new enterovirus isolated from patients with disease of the central nervous system. J Infect Dis 129:304–309
    [Google Scholar]
  15. Shindarov L. M., Chumakov M. P., Voroshilova M. K. 10 other authors 1979; Epidemiological, clinical and patholomorphological characteristics of epidemic poliomyelitis-like disease caused by enterovirus 71. J Hyg Epid Microbiol Immunol 23:284–295
    [Google Scholar]
  16. Wang S. M., Liu C. C., Tseng H. W., Wang J. R., Huang C. C., Chen Y. J., Yang Y. J., Lin S. J., Yeh T. F. 1999; Clinical spectrum of enterovirus 71 infection of children in southern Taiwan, with an emphasis on the neurological complications. Clin Infect Dis 29:184–190
    [Google Scholar]
  17. Wen Y. Y., Chang T. Y., Chen S.-T., Li C., Liu H. S. 2003; Comparative study of enterovirus 71 infection of human cell lines. J Med Virol 70:109–118
    [Google Scholar]
  18. Wu C. N., Lin Y. C., Fann C., Liao N. S., Shih S. R., Ho M. S. 2001; Protection against lethal enterovirus 71 infection in newborn mice by passive immunization with subunit VP1 vaccines and inactivated virus. Vaccine 20:895–904
    [Google Scholar]
  19. Wu J. M., Wang J. N., Tsai Y. C., Liu C. C., Huang C. C., Chen Y. J., Yeh T. F. 2002; Cardiopulmonary manifestations of fulminant enterovirus 71 infection. Pediatrics 109:e26
    [Google Scholar]
  20. Yang W. X., Terasaki T., Shiroki K. 7 other authors 1997; Efficient delivery of circulating poliovirus to the central nervous system independently of poliovirus receptor. Virology 229:421–428
    [Google Scholar]
  21. Yang J. J., Su I. J., Chen P. F., Liu C. C., Yu C. K., Wang J. R. 2001; Complete genome analysis of enterovirus 71 isolated from an outbreak in Taiwan and rapid identification of enterovirus 71 and coxsackievirus A16 by RT-PCR. J Med Virol 65:331–339
    [Google Scholar]
  22. Yu C. K., Chen C. C., Chen C. L., Wang J. R., Liu C. C., Yan J. J., Su I. J. 2000; Neutralizing antibody provided protection against enterovirus type 71 lethal challenge in neonatal mice. J Biomed Sci 7:523–528
    [Google Scholar]
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